A novel protein-repellent dental composite containing 2-methacryloyloxyethyl phosphorylcholine.
A novel protein-repellent dental composite containing 2-methacryloyloxyethyl phosphorylcholine.
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DOI：
10.1038/ijos.2014.77
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发表时间：
2015-06-26
影响因子：
14.9
通讯作者：
Xu HH
中科院分区：
文献类型：
--
作者：
Zhang N;Chen C;Melo MA;Bai YX;Cheng L;Xu HH
生物膜酸引起的继发龋是牙科复合材料修复失败的主要原因。迄今为止，还没有牙科复合材料能够排斥蛋白质吸附和抑制细菌附着的报道。本研究的目的是通过掺入 2-甲基丙烯酰氧基乙基磷酰胆碱 (MPC) 开发一种蛋白质排斥性牙科复合材料，并首次研究 MPC 质量分数对蛋白质吸附、细菌附着、生物膜生长和机械性能的影响。合成复合材料时，MPC 质量分数为 0（对照）、0.75%、1.5%、2.25%、3%、4.5% 和 6%。还测试了商业复合材料作为对照。机械性能通过三点弯曲进行测量。通过微生物二辛可宁酸法测定复合物上的蛋白质吸附。使用人类唾液微观生物膜模型。研究了 4 小时的早期附着、2 天的生物膜、活/死染色和复合材料上生长的生物膜的集落形成单位 (CFU)。 MPC 含量高达 3% 的复合材料的机械性能与不含 MPC 的复合材料和商业对照的机械性能相似，而 4.5% 和 6% MPC 则降低了机械性能（P<0.05）。 MPC 从 0% 增加到 3% 降低了复合材料上的蛋白质吸附 (P<0.05)。含3%MPC的复合材料的蛋白质吸附量是对照的1/12（P<0.05）。与对照相比，含有 3% MPC 的复合材料的口腔细菌早期附着和生物膜生长也大大减少（P<0.05）。总之，在复合材料中加入 3% 的 MPC 可以大大减少蛋白质吸附、细菌附着和生物膜 CFU，同时不会影响机械性能。蛋白质排斥复合材料有助于排斥细菌附着和牙菌斑积聚，从而减少继发龋。蛋白质排斥方法可能适用于其他牙科材料。
Secondary caries due to biofilm acids is a primary cause of dental composite restoration failure. To date, there have been no reports of dental composites that can repel protein adsorption and inhibit bacteria attachment. The objectives of this study were to develop a protein-repellent dental composite by incorporating 2-methacryloyloxyethyl phosphorylcholine (MPC) and to investigate for the first time the effects of MPC mass fraction on protein adsorption, bacteria attachment, biofilm growth, and mechanical properties. Composites were synthesized with 0 (control), 0.75%, 1.5%, 2.25%, 3%, 4.5% and 6% of MPC by mass. A commercial composite was also tested as a control. Mechanical properties were measured in three-point flexure. Protein adsorption onto the composite was determined by the microbicinchoninic acid method. A human saliva microcosm biofilm model was used. Early attachment at 4 h, biofilm at 2 days, live/dead staining and colony-forming units (CFUs) of biofilms grown on the composites were investigated. Composites with MPC of up to 3% had mechanical properties similar to those without MPC and those of the commercial control, whereas 4.5% and 6% MPC decreased the mechanical properties (P<0.05). Increasing MPC from 0 to 3% reduced the protein adsorption on composites (P<0.05). The composite with 3% MPC had protein adsorption that was 1/12 that of the control (P<0.05). Oral bacteria early attachment and biofilm growth were also greatly reduced on the composite with 3% MPC, compared to the control (P<0.05). In conclusion, incorporation of MPC into composites at 3% greatly reduced protein adsorption, bacteria attachment and biofilm CFUs, without compromising mechanical properties. Protein-repellent composites could help to repel bacteria attachment and plaque build-up to reduce secondary caries. The protein-repellent method might be applicable to other dental materials.