Solution properties of synthetic chlorophyllide--and bacteriochlorophyllide--apomyoglobin complexes.
Solution properties of synthetic chlorophyllide--and bacteriochlorophyllide--apomyoglobin complexes.
复制标题
合成叶绿素内酯-和细菌叶绿素内酯-脱肌红蛋白复合物的溶液特性。
DOI：
10.1021/bi00529a033
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发表时间：
1981
期刊：
影响因子：
2.9
通讯作者：
Boxer,SG
中科院分区：
文献类型：
--
作者：
Wright,KA;Boxer,SG
Karen A.Wright和Steven G.Boxer*摘要：无肌红蛋白(ApoMb)与一些叶绿素类化合物形成了明确的1：1络合物。在肌红蛋白的口袋中，叶绿素取代了血红素。这些化合物包括氯代叶绿素a镁、镁和锌焦叶绿素a、焦叶绿素b锌、焦叶绿素d锌、焦中叶绿素a锌、2-乙酰基-2-去乙烯基叶绿素a锌、原焦叶绿素a锌和锌细菌叶绿内酯a，比较了蛋白质在有机溶剂中对电子吸收、圆二色谱、磁性圆二色谱、三态电子自旋共振光谱和荧光寿命的影响。除CD光谱外，蛋白质引起的位移和强度变化在溶剂效应观察到的范围内。CD光谱发生了很大的变化：几个转变的符号在氯素中完全颠倒了，并且观察到细菌叶绿素锌的强度增加了3-6倍
Karen A. Wright and Steven G. Boxer* abstract: Well-defined 1: 1 complexes have been formed between apomyoglobin (apoMb) and a number of chlorophyllide derivatives. The chlorophyllides substitute for heme in the pocket of myoglobin. These include magnesium chlo-rophyllide a, magnesium and zinc pyrochlorophyllide a, zinc pyrochlorophyllide b, zinc pyrochlorophyllide d, zinc pyro-mesochlorophyllide a, zinc 2-acetyl-2-devinylpyrochlorophyllide a, zinc protopyrochlorophyllide a, and zinc bacteriopyrochlorophyllide a. The effects of the protein on the electronic absorption, circular dichroism (CD), magnetic circular dichroism, and triplet state electron spin resonance spectra and fluorescence lifetimes in solution are compared with appropriate models in organic solvents. With the exception of the CD spectra, the protein causes shifts and intensity changes which are within the range observed for solvent effects. The CD spectra change substantially: the signs of several transitions are entirely reversed in the chlorins, and 3-6-fold intensity increases are observed with zinc bacteriochlorophyllide