Application of capillary zone electrophoresis in the separation and determination of the curcuminoids in urine
Application of capillary zone electrophoresis in the separation and determination of the curcuminoids in urine
复制标题
毛细管区带电泳在尿液中姜黄素类化合物分离测定中的应用
DOI：
10.1016/j.jpba.2004.12.009
复制
发表时间：
2005-06-01
影响因子：
3.4
通讯作者：
Xu, GW
中科院分区：
文献类型：
--
作者：
Yuan, KL;Weng, QF;Xu, GW
植物姜黄的主要成分是姜黄素类化合物，包括姜黄素、脱甲氧基姜黄素和双脱甲氧基姜黄素。姜黄素类化合物具有重要的生物活性。建立了一种新的毛细管区带电泳-二极管阵列检测法分离测定姜黄素、去甲氧基姜黄素和双去甲氧基姜黄素。在pH10.8，25 kV和30 ℃下，用含10%甲醇（v/v）的15 mM四硼酸钠缓冲液在7 min内可容易地分离三种姜黄素。该方法已经过验证，在选择性、重现性、线性、检测限和回收率方面表现良好。该方法已成功应用于尿液中姜黄素类化合物的测定。(c)2004 Elsevier B. V.保留所有权利。
The major components of the plant curcuma longa are the curcuminoids that include curcumin, demethoxycurcumin and bisdemethoxycurcumin. It has been reported the curcuminoids have some important activities. A new CZE method with diode array detection has been developed for the separation and determination of the curcumin, demethoxycurcumin and bisdemethoxycurcumin. Three curcuminoids could be readily separated within 7 min with a 15 mM sodium tetraborate buffer containing 10% methanol (v/v) at pH 10.8, 25 kV and 30 degrees C. The method has been validated and shows good performance with respect to selectivity, reproducibility, linearity, limits of detection and recovery. The proposed method was successfully applied to determine the curcuminoids in urine. (c) 2004 Elsevier B.V. All rights reserved.