A WIDELY EXPRESSED HUMAN PROTEIN-TYROSINE PHOSPHATASE CONTAINING SRC HOMOLOGY-2 DOMAINS
A WIDELY EXPRESSED HUMAN PROTEIN-TYROSINE PHOSPHATASE CONTAINING SRC HOMOLOGY-2 DOMAINS
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DOI：
10.1073/pnas.90.6.2197
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发表时间：
1993-03-15
影响因子：
11.1
通讯作者：
SHEN, SH
中科院分区：
文献类型：
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作者：
AHMAD, S;BANVILLE, D;SHEN, SH
从人脐带cDNA文库中分离出编码非跨膜蛋白酪氨酸磷酸酶（PTP; EC 3.1.3.48）的cDNA，称为PTP 2C。该酶含有一个磷酸酶结构域和两个相邻拷贝的src同源2（SH 2）结构域在其氨基末端。还鉴定了在催化结构域内具有四个额外氨基酸残基的PTP 2C（PTP 2Ci）的变体。PTP 2C在人体组织中广泛表达，在心脏、脑和骨骼肌中尤其丰富。PTP 2C的催化结构域在大肠杆菌中表达为重组酶，并通过两个色谱步骤纯化至接近均一。重组酶对磷酸酪氨酸残基具有完全特异性。PTP 2C和先前描述的PTP 1C之间的结构相似性表明存在含SH 2的PTPs的亚家族;这些可能通过其SH 2结构域与含磷酸酪氨酸的蛋白质的相互作用在信号转导中发挥重要作用。
A cDNA encoding a nontransmembrane protein-tyrosine phosphatase (PTP; EC 3.1.3.48), termed PTP2C, was isolated from a human umbilical cord cDNA library. The enzyme contains a single phosphatase domain and two adjacent copies of the src homology 2 (SH2) domain at its amino terminus. A variant of PTP2C (PTP2Ci) which has four extra amino acid residues within the catalytic domain has been identified also. PTP2C is widely expressed in human tissues and is particularly abundant in heart, brain, and skeletal muscle. The catalytic domain of PTP2C was expressed as a recombinant enzyme in Escherichia coli and purified to near homogeneity by two chromatographic steps. The recombinant enzyme was totally specific toward phosphotyrosine residues. The structural similarity between PTP2C and the previously described PTP1C suggests the existence of a subfamily of SH2-containing PTPs; these may play an important role in signal transduction through interaction of their SH2 domains with phosphotyrosine-containing proteins.