Migration of Neuronal Precursors From the Telencephalic Ventricular Zone Into the Olfactory Bulb in Adult Zebrafish
Migration of Neuronal Precursors From the Telencephalic Ventricular Zone Into the Olfactory Bulb in Adult Zebrafish
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DOI：
10.1002/cne.22722
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发表时间：
2011-12-01
影响因子：
2.5
通讯作者：
Sawamoto, Kazunobu
中科院分区：
文献类型：
--
作者：
Kishimoto, Norihito;Alfaro-Cervello, Clara;Sawamoto, Kazunobu
在成年哺乳动物的脑中，神经元前体产生于侧脑室侧壁的脑室下区，并通过被称为吻侧迁移流（RMS）的充分研究的路线迁移到嗅球（OB）中。最近的研究表明，一个类似的神经干细胞龛广泛保存在成年脊椎动物的心室壁。然而，很少有人知道在非哺乳动物的成年大脑中的神经元前体的迁移路线。在这里，我们表明，在成年斑马鱼，在端脑脑室区产生的神经元前体集群迁移到OB通过相当于哺乳动物RMS的路线。与哺乳动物RMS不同，这些神经元前体不被神经胶质管包围，尽管与胚胎和新生哺乳动物一样，端脑心室壁有放射状胶质细胞，具有单一纤毛。为了观察活体脑组织中迁移的神经元前体，我们使用携带由神经生成素1（ngn 1）启动子驱动的GFP转基因的斑马鱼系建立了脑半球培养物。在这些鱼中，在RMS中迁移的神经元前体中观察到GFP，其中一些与血管对齐。大量ngn 1：gfp阳性细胞在OB内侧RMS样路径中切向迁移。综上所述，我们的研究结果表明，RMS在成年斑马鱼端脑是一个功能性的迁移途径。这是非哺乳动物成人端脑神经元前体切向迁移的第一个证据。神经学比较杂志519：3549-3565，2011。(C)2011 Wiley-Liss，Inc.
In the brain of adult mammals, neuronal precursors are generated in the subventricular zone in the lateral wall of the lateral ventricles and migrate into the olfactory bulbs (OBs) through a well-studied route called the rostral migratory stream (RMS). Recent studies have revealed that a comparable neural stem cell niche is widely conserved at the ventricular wall of adult vertebrates. However, little is known about the migration route of neuronal precursors in nonmammalian adult brains. Here, we show that, in the adult zebrafish, a cluster of neuronal precursors generated in the telencephalic ventricular zone migrates into the OB via a route equivalent to the mammalian RMS. Unlike the mammalian RMS, these neuronal precursors are not surrounded by glial tubes, although radial glial cells with a single cilium lined the telencephalic ventricular wall, much as in embryonic and neonatal mammals. To observe the migrating neuronal precursors in living brain tissue, we established a brain hemisphere culture using a zebrafish line carrying a GFP transgene driven by the neurogenin1 (ngn1) promoter. In these fish, GFP was observed in the neuronal precursors migrating in the RMS, some of which were aligned with blood vessels. Numerous ngn1:gfp-positive cells were observed migrating tangentially in the RMS-like route medial to the OB. Taken together, our results suggest that the RMS in the adult zebrafish telencephalon is a functional migratory pathway. This is the first evidence for the tangential migration of neuronal precursors in a nonmammalian adult telencephalon. J. Comp. Neurol. 519:3549-3565, 2011. (C) 2011 Wiley-Liss, Inc.