Effects of blood sample handling procedures on measurable interleukin 6 in plasma and serum
Effects of blood sample handling procedures on measurable interleukin 6 in plasma and serum
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DOI：
10.1002/jcla.22924
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发表时间：
2019-09-01
影响因子：
2.7
通讯作者：
Yuan, Xiaopeng
中科院分区：
文献类型：
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作者：
Gong, Yan;Liang, Shaocong;Yuan, Xiaopeng
白细胞介素-6（IL-6）的测量在医学诊断、治疗和各种疾病的预后中用作生物标志物。然而，一些预分析因素可能会产生错误的IL-6结果。在这项研究中，我们着手研究校正的血液样本处理程序对可测量的IL-6的影响。方法收集45例健康人EDTA血浆和血清标本。参与者被分成三组，执行不同的处理程序。对样品执行不同的离心时间、储存温度和时间。分析IL-6水平的变化趋势。结果基线时，血浆IL-6与血清IL-6配对值具有良好的相关性，但血浆水平高于血清水平。一般情况下，未分离的EDTA血浆随时间保持稳定。随着储存温度和时间的增加，观察到未分离的血清IL-6的更明显的上升。然而，第3组中的样品经离心和分离后立即在不同温度和长时间储存后保持稳定。结论标本类型、离心时间、保存温度和时间可能影响IL-6水平。应执行标准血液样本处理程序，以确保IL-6值的准确性和稳定性。
Introduction Interleukin-6(IL-6) measurement is used as a biomarker in medical diagnosis, therapy, and prognosis in various diseases. However, several pre-analytical factors may yield a false IL-6 result. In this study, we set out to investigate the effects of corrected blood sample handling procedures on measurable IL-6. Method EDTA plasma and serum samples were collected from 45 healthy individuals. The participants were divided into three groups to perform different handling procedures. Different centrifugal timing, storage temperature, and time were executed on the samples. The changed trends of IL-6 levels were analyzed. Results At baseline, while the paired plasma and serum IL-6 values had a good correlation, the plasma levels were higher than serum. In general, the unseparated EDTA plasma kept steady with time. With the increase in storage temperature and time, a more pronounced rise in unseparated serum IL-6 was observed. Nevertheless, the samples in Group 3 which centrifuged and separated immediately kept stable after a different temperature and longtime storage. Conclusion Sample types, centrifugal timing, storage temperature, and time may affect the IL-6 levels. A standard blood sample handling procedure should be performed to ensure the accuracy and stability of IL-6 values.