A 3D hydrodynamic flow-focusing device for cell sorting
A 3D hydrodynamic flow-focusing device for cell sorting
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DOI：
10.1007/s10404-021-02425-y
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发表时间：
2021-02
影响因子：
2.8
通讯作者：
Xiaofei Yuan;A. Glidle;H. Furusho;Huabing Yin
中科院分区：
文献类型：
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作者：
Xiaofei Yuan;A. Glidle;H. Furusho;Huabing Yin
基于光学的微流控细胞分选由于其在流动中复杂的细胞处理能力而在生命和环境科学中的应用变得越来越有吸引力。这些微流体细胞分选装置中的大多数采用二维流体流动控制策略，其缺乏任意操纵细胞位置以进行精确光学检测的能力，因此导致分选准确性和纯度降低。虽然三维（3D）流体动力装置具有更好的流动聚焦特性，但大多数缺乏在每个方向上任意定位样品流的灵活性。因此，使用3D流体动力学流聚焦进行分选的研究很少。为此，我们设计了一种基于独立鞘流聚焦和压力驱动切换的三维流体动力聚焦分选平台。由于能够精确控制样品在3D中的速度和位置，该设计在弱拉曼信号的可靠采集方面提供了许多优点。通过概念验证演示，我们展示了这种基于3D流体动力学聚焦的分选设备具有达到高精度的拉曼激活分选的潜力。
Optical-based microfluidic cell sorting has become increasingly attractive for applications in life and environmental sciences due to its ability of sophisticated cell handling in flow. The majority of these microfluidic cell sorting devices employ two-dimensional fluid flow control strategies, which lack the ability to manipulate the position of cells arbitrarily for precise optical detection, therefore resulting in reduced sorting accuracy and purity. Although three-dimensional (3D) hydrodynamic devices have better flow-focusing characteristics, most lack the flexibility to arbitrarily position the sample flow in each direction. Thus, there have been very few studies using 3D hydrodynamic flow focusing for sorting. Herein, we designed a 3D hydrodynamic focusing sorting platform based on independent sheath flow-focusing and pressure-actuated switching. This design offers many advantages in terms of reliable acquisition of weak Raman signals due to the ability to precisely control the speed and position of samples in 3D. With a proof-of-concept demonstration, we show this 3D hydrodynamic focusing-based sorting device has the potential to reach a high degree of accuracy for Raman activated sorting.