Elevated Pressure Improves the Rate of Formalin Penetration while Preserving Tissue Morphology.
Elevated Pressure Improves the Rate of Formalin Penetration while Preserving Tissue Morphology.
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DOI：
10.7150/jca.1.178
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发表时间：
2010-10-12
影响因子：
3.9
通讯作者：
Fowler CB
中科院分区：
文献类型：
--
作者：
Chesnick IE;Mason JT;O'Leary TJ;Fowler CB
甲醛固定和石蜡包埋仍然是用于病理检查、组织形态学研究和档案保存的肿瘤组织标本处理中最广泛使用的技术。然而，甲醛渗透和固定是一个缓慢的过程，病理样品的常规处理至少需要15小时。常规固定的样品通常具有固定良好的外缘，而组织的内芯固定较差。在这项研究中，我们表明，应用高达15,000 psi的高压可将甲醛固定率提高约5至7倍，同时保留猪肝的组织形态。与在大气压下固定相同时间的样品相比，在高压下培养30-60分钟后，组织也表现出更均匀的甲醛渗透。
Formaldehyde fixation and paraffin-embedding remains the most widely used technique for processing cancer tissue specimens for pathologic examination, the study of tissue morphology, and archival preservation. However, formaldehyde penetration and fixation is a slow process, requiring a minimum of 15 hr for routine processing of pathology samples. Routinely fixed samples often have a well-fixed outer rim, with a poorly-fixed inner core of tissue. In this study, we show that the application of elevated pressure up to 15,000 psi improves the rate of formaldehyde fixation by approximately 5 to 7-fold while preserving the tissue morphology of porcine liver. The tissue also exhibited much more uniform formaldehyde penetration after 30-60 min incubation under elevated pressure than samples fixed for the same length of time at atmospheric pressure.