FUNCTIONAL-ORGANIZATION OF CHLOROPHYLL-ALPHA AND CAROTENOIDS IN THE ALGA, NANNOCHLOROPSIS-SALINA
FUNCTIONAL-ORGANIZATION OF CHLOROPHYLL-ALPHA AND CAROTENOIDS IN THE ALGA, NANNOCHLOROPSIS-SALINA
复制标题
DOI：
10.1104/pp.83.2.434
复制
发表时间：
1987-02-01
期刊：
影响因子：
7.4
通讯作者：
BROWN, JS
中科院分区：
文献类型：
--
作者：
BROWN, JS
从一种黄绿色的盐藻微拟球藻（Nannochloropsis salina）中分离出叶绿素-蛋白质复合物。获得了三种不同的复合物，对应于三种主要的叶绿素蛋白分离菠菜叶绿体通过相同的程序和先前确定为反应中心复合物的光系统I和II和捕光复合物。从它们的吸收和荧光光谱以及相对色素含量得出藻类复合物与菠菜复合物之间的相似性。通过HPLC测定与每个分离的复合物相关的主要类胡萝卜素的身份和量。虽然反应中心复合物仅占总叶绿素的14%，但它们高度富集β-胡萝卜素，而捕光复合物含有高比例的叶黄素（主要是紫黄质和vaucheriaxanthin酯）。藻膜的荧光激发光谱表明，一个或两个主要的叶黄素可能作为天线色素的光合作用。
Chlorophyll-protein complexes were isolated from a yellow-green alga, Nannochloropsis salina after mild detergent treatment and gel electrophoreis. Three different complexes were obtained which correspond to the three major kinds of chlorophyll-proteins isolated from spinach chloroplasts by the same procedure and previously identified as reaction center complexes for photosystems I and II and a light-harvesting complex. The analogy between the algal complexes and those from spinach was drawn from their absorption and fluoresence spectra and relative pigment content. The identities and amounts of the major carotenoids associated with each isolated complex were determined by HPLC. Although the reaction center complexes accounted for only 14% of the total chlorophyll, they were highly enriched in .beta.-carotene, whereas the light-harvesting complex contained a high proportion of xanthophylls (mainly violaxanthin and vaucheriaxanthin-ester). Fluorescence excitation spectra of the algal membranes showed that one or both of the major xanthophylls may act as antenna pigment for photosynthesis.