Transcriptome differences between Cupriavidus necator NH9 grown with 3-chlorobenzoate and that grown with benzoate
Transcriptome differences between Cupriavidus necator NH9 grown with 3-chlorobenzoate and that grown with benzoate
复制标题
使用 3-氯苯甲酸盐和苯甲酸盐生长的 Cupriavidus necator NH9 之间的转录组差异
DOI：
10.1093/bbb/zbab044
复制
发表时间：
2021
期刊：
影响因子：
--
通讯作者：
Naoto Ogawa
中科院分区：
文献类型：
--
作者：
Ryota Moriuchi;Hideo Dohra;Yu Kanesaki;Naoto Ogawa
用3-氯苯甲酸盐和苯甲酸盐培养的3-氯苯甲酸盐降解细菌钩虫贪铜菌NH 9的RNA-seq分析揭示了在相应化合物的降解途径中编码酶的基因的强烈诱导，包括将3-氯苯甲酸盐和苯甲酸盐分别转化为氯邻苯二酚和邻苯二酚的基因，以及用于转化为中心代谢物的氯邻苯二酚邻位裂解途径的基因。编码转运蛋白、应激反应组分、鞭毛蛋白和趋化蛋白的基因在3-氯苯甲酸和苯甲酸之间表现出改变的表达模式。基因本体富集分析表明，趋化性相关的条款显着上调苯甲酸相比，3-氯苯甲酸。与此一致，在半固体琼脂平板测定中，NH 9细胞对苯甲酸盐的趋化性比对3-氯苯甲酸盐的趋化性强。这些结果，结合与3-氯苯甲酸盐的吸收/趋化性相关的基因的缺失，与3-氯苯甲酸盐的降解基因接近，表明NH 9在自然界中没有完全适应氯代苯甲酸盐的利用，与苯甲酸盐不同。
RNA-seq analysis ofCupriavidus necatorNH9, a 3-chlorobenzoate degradative bacterium, cultured with 3-chlorobenzaote and benzoate, revealed strong induction of genes encoding enzymes in degradation pathways of the respective compound, including the genes to convert 3-chlorobenzaote and benzoate to chlorocatechol and catechol, respectively, and the genes of chlorocatecholortho-cleavage pathway for conversion to central metabolites. The genes encoding transporters, components of the stress response, flagellar proteins, and chemotaxis proteins showed altered expression patterns between 3-chlorobenzoate and benzoate. Gene Ontology enrichment analysis revealed that chemotaxis-related terms were significantly upregulated by benzoate compared with 3-chlorobenzoate. Consistent with this, in semisolid agar plate assays, NH9 cells showed stronger chemotaxis to benzoate than to 3-chlorobenzoate. These results, combined with the absence of genes related to uptake/chemotaxis for 3-chlorobenzoate located closely to the degradation genes of 3-chlorobenzoate, suggested that NH9 has not fully adapted to the utilization of chlorinated benzoate, unlike benzoate, in nature.