Evaluation of the Protective Activities of a Late Embryogenesis Abundant (LEA) Related Protein, Cor15am, during Various Stresses in Vitro
Evaluation of the Protective Activities of a Late Embryogenesis Abundant (LEA) Related Protein, Cor15am, during Various Stresses in Vitro
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DOI：
10.1271/bbb.80214
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发表时间：
2008-06
期刊：
影响因子：
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通讯作者：
K. Nakayama;Kumiko Okawa;T. Kakizaki;T. Inaba
中科院分区：
文献类型：
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作者：
K. Nakayama;Kumiko Okawa;T. Kakizaki;T. Inaba
拟南芥 Cor15am 是一种晚期胚胎发生丰富 (LEA) 相关蛋白，已被证明在体外表现出冷冻保护活性。在这项研究中，我们进一步研究了 Cor15am 保护底物免于失活的机制。尽管 Cor15am 不表现出重折叠活性，但它在体外表现出针对各种应激的保护活性。这可能归因于 Cor15am 减弱底物聚集的活性。我们的数据表明，Cor15am 通过防止蛋白质聚集而发挥保护剂的作用，抵御各种压力。
Arabidopsis Cor15am is a late embryogenesis abundant (LEA) related protein that has been shown to exhibit cryoprotective activity in vitro. In this study, we further investigated the mechanisms by which Cor15am protects substrates from inactivation. Although Cor15am did not exhibit refolding activity, it showed protective activity against various stresses in vitro. This might be attributable to the activity of Cor15am in attenuating the aggregation of the substrates. Our data indicate that Cor15am functions as a protectant against various stresses by preventing protein aggregation.