A new strain of Butyrivibrio fibrisolvens that has high ability to isomerize linoleic acid to conjugated linoleic acid
A new strain of Butyrivibrio fibrisolvens that has high ability to isomerize linoleic acid to conjugated linoleic acid
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DOI：
10.2323/jgam.51.105
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发表时间：
2005-04-01
影响因子：
1.2
通讯作者：
Hino, T
中科院分区：
文献类型：
--
作者：
Fukuda, S;Furuya, H;Hino, T
分离到一株具有高产共轭亚油酸（CLA）潜力的溶纤维丁酸弧菌（TH 1）新菌株。菌株TH 1具有较高的LA异构酶（LA-Ⅰ）活性，且对亚油酸（LA）的耐受性比其它菌株高。然而，高CLA还原酶（CLA-R）活性导致CLA的暂时积累和随后转化为反式异油酸（t-VA）。当LA被添加到生长的TH 1培养物中的二甲基亚砜溶液（LA/DMSO），产生的CLA大于当LA被添加到与牛血清白蛋白（BSA）的混合物中。加入LA/DMSO后，活细胞数减少，但随后随着CLA转化为t-VA后的减少而增加。这一结果表明，B。解纤蛋白可以通过从细胞中除去CLA而恢复生长。大部分CLA从B释放。通过用BSA温和洗涤，表明结合到细胞上的CLA可能在瘤胃和大肠中被去除。因此，通过B.消化道中的溶纤蛋白可通过CLA-R活性的降低而增加，而不伴随B细胞数量的总体减少。纤维溶剂18碳脂肪酸（FAs）诱导LA I活性，而不饱和脂肪酸诱导LA R活性，表明FAs刺激LA I和LA R的合成。提供具有低比例的不饱和脂肪酸与饱和脂肪酸的饮食可能有利于CLA的产生。
A new strain of Butyrivibrio fibrisolvens (TH1) that has high potential to produce conjugated linoleic acid (CLA) was isolated. Strain TH1 had higher LA isomerase (LA-I) activity, and was much more tolerant to linoleic acid (LA) than other strains examined. However, high CLA reductase (CLA-R) activity resulted in the temporary accumulation of CLA and subsequent conversion to trans-vaccenic acid (t-VA). When LA was added to growing TH1 cultures in a solution with dimethylsulfoxide (LA/DMSO), CLA produced was greater than when LA was added in a mixture with bovine serum albumin (BSA). The number of viable cells decreased upon addition of LA/DMSO, but then increased as the CLA decreased upon its conversion to t-VA. This result suggests that B. fibrisolvens can resume growing by the removal of CLA from the cells. Most CLA was released from B. fibrisolvens cells by gentle washing with BSA, suggesting that CLA bound to the cells might be removed in the rumen and large intestine. Thus, CLA production by B. fibrisolvens in the digestive tract could be increased by a reduction in CLA-R activity without accompanying an overall decrease in the cell number of B. fibrisolvens. Fatty acids (FAs) with 18 carbon backbone inducted LA-I activity, whereas unsaturated FAs induced CLA-R activity, suggesting that FAs stimulate the synthesis of LA-I and CLA-R. Providing a diet with a low ratio of unsaturated to saturated FAs may favor CLA production.