Isolation of the β-galactosphingolipid coniferoside using a tumor cell proteome reverse affinity protocol
Isolation of the β-galactosphingolipid coniferoside using a tumor cell proteome reverse affinity protocol
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DOI：
10.1016/j.bmc.2011.06.051
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发表时间：
2011-11-15
影响因子：
3.5
通讯作者：
Rodriguez, Abimael D.
中科院分区：
文献类型：
--
作者：
La Clair, James J.;Rodriguez, Abimael D.
新方法对于开发海洋天然产物(MNP)作为治疗先导至关重要。MNP研究的一个更耗时的方面是结构和功能之间的联系。在这里，我们描述了一种分离方案，该方案将肿瘤细胞蛋白质组作为载体，结合结构和功能分析分离其中的MNP。将该方法应用于松柏海绵的提取，得到了一种独特的聚羟基丁基β-半乳糖脂，即松柏糖苷。(C)爱思唯尔有限公司出版的2011年。
New approaches are vital to the development of marine natural products (MNP) as therapeutic leads. One of the more time consuming aspects of MNP research arises in the connection between structure and function. Here, we describe an isolation protocol that adapts tumor cell proteomes as a vehicle for MNP isolation therein uniting structural and functional analysis. Application of this method to extracts of the sponge Agelas conifera led to the isolation of a unique poly-hydroxybutyrated beta-galactosphingolipid, coniferoside. (C) 2011 Published by Elsevier Ltd.