Detergent-Free Incorporation of a Seven-Transmembrane Receptor Protein into Nanosized Bilayer Lipodisq Particles for Functional and Biophysical Studies
Detergent-Free Incorporation of a Seven-Transmembrane Receptor Protein into Nanosized Bilayer Lipodisq Particles for Functional and Biophysical Studies
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DOI：
10.1021/nl3020395
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发表时间：
2012-09-01
期刊：
影响因子：
10.8
通讯作者：
Watts, Anthony
中科院分区：
文献类型：
--
作者：
Orwick-Rydmark, Marcella;Lovett, Janet E.;Watts, Anthony
SMA-Lipodisq纳米颗粒，平均每12 nm颗粒具有一个细菌视紫红质（bR）（蛋白质/脂质摩尔比，1：172），在不使用去污剂的情况下制备。使用脉冲和连续波氮氧自由基自旋标记电子顺磁共振，与天然膜和洗涤剂中获得的bR数据以及晶体数据相比，bR的结构和动力学完整性得到保留。这表明Lipodisq纳米颗粒作为有用的膜模拟物的潜力。
SMA-Lipodisq nanoparticles, with one bacteriorhodopsin (bR) per 12 nm particle on average (protein/lipid molar ratio, 1:172), were prepared without the use of detergents. Using pulsed and continuous wave nitroxide spin label electron paramagnetic resonance, the structural and dynamic integrity of bR was retained when compared with data for bR obtained in the native membrane and in detergents and then with crystal data. This indicates the potential of Lipodisq nanoparticles as a useful membrane mimetic.