Fetal grafts containing suprachiasmatic nuclei restore the diurnal rhythm of CRH and POMC mRNA in aging rats
Fetal grafts containing suprachiasmatic nuclei restore the diurnal rhythm of CRH and POMC mRNA in aging rats
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DOI：
10.1152/ajpregu.1997.273.5.r1764
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发表时间：
1997-11-01
影响因子：
2.8
通讯作者：
Wise, PM
中科院分区：
文献类型：
--
作者：
Cai, AH;Scarbrough, K;Wise, PM
我们评估了含有视交叉上核（SCN）的胎儿组织是否能够恢复下丘脑促肾上腺皮质激素释放激素（CRH）和垂体前叶阿黑皮素原（POMC）mRNA昼夜节律的年龄相关变化。年轻、中年以及接受了SCN移植的中年大鼠在一天中的七个不同时间被处死。在年轻大鼠中，CRH mRNA在背内侧室旁核呈现昼夜节律，但在该核的其他分区则没有。在老年大鼠中未检测到节律。SCN移植恢复了CRH mRNA的节律，但时间与年轻动物并不完全相同。POMC mRNA在年轻大鼠中呈现每日节律。衰老消除了该节律并降低了平均mRNA水平；胎儿移植恢复了节律，但幅度仍然减弱。这些数据首次证明胎儿组织能够恢复由SCN驱动的神经内分泌轴的昼夜节律。我们得出结论，当胎儿SCN移植提供适当信号时，来自衰老宿主的神经内分泌底物仍然能够对昼夜信号作出反应，以表达CRH/POMC mRNA的昼夜节律性。
We assessed whether fetal tissue containing the suprachiasmatic nuclei (SCN) can restore age-related changes in the diurnal rhythm of hypothalamic corticotropin-releasing hormone (CRH) and anterior pituitary proopiomelanocortin (POMC) mRNA. Young, middle-aged, and middle-aged SCN-transplanted rats were killed at seven times of day. In young rats, CRH mRNA exhibited a diurnal rhythm in the dorsomedial paraventricular nuclei but not in other subdivisions of the nuclei. No rhythm was detected in aging rats. SCN transplants restored a rhythm in CRH mRNA, but the timing was not precisely the same as in young animals. POMC mRNA exhibited a daily rhythm in young rats. Aging abolished the rhythm and decreased the average mRNA level; fetal transplants restored the rhythm, but the amplitude remained attenuated. These data are the first demonstration that fetal tissue can restore the diurnal rhythm of a neuroendocrine axis that is driven by the SCN. We conclude that the neuroendocrine substrate from the aging host remains capable of responding to diurnal cues to express diurnal rhythmicity in CRH/POMC mRNA when fetal SCN transplants confer the appropriate signals.