Microbubble size isolation by differential centrifugation
Microbubble size isolation by differential centrifugation
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DOI：
10.1016/j.jcis.2008.09.066
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发表时间：
2009-01-15
影响因子：
9.9
通讯作者：
Borden, Mark A.
中科院分区：
文献类型：
--
作者：
Feshitan, Jameel A.;Chen, Cherry C.;Borden, Mark A.
用作超声成像造影剂、靶向药物递送载体和代谢气体运输载体的微泡需要更好的尺寸控制以提高性能。机械搅拌是目前唯一可用的方法，以产生足够的产量用于生物医学应用的微泡，但乳液往往是多分散的。在此，我们描述了一项研究，以产生脂质涂层，全氟丁烷填充的微泡和隔离其大小的分数的基础上迁移在离心场。通过光阻/散射和电阻抗传感、荧光和明场显微镜和流式细胞术，通过颗粒大小测定和计数来表征新鲜超声处理的悬浮液的多分散性。我们发现，大小分布是多峰的。更小的微泡更丰富。采用差速离心法成功地分离出直径为1-2和4-5 μ m的级分。分离的微泡在两天内是稳定的。然而，两周后，更稀的悬浮液（
Microbubbles used as contrast agents for ultrasound imaging, vectors for targeted drug delivery and vehicles for metabolic gas transport require better size control for improved performance. Mechanical agitation is the only method currently available to produce microbubbles in sufficient yields for biomedical applications, but the emulsions tend to be polydisperse. Herein, we describe a study to generate lipid-coated, perfluorobutane-filled microbubbles and isolate their size fractions based on migration in a centrifugal field. Polydispersity of the freshly sonicated suspension was characterized by particle sizing and counting through light obscuration/scattering and electrical impedance sensing, fluorescence and bright-field microscopy and flow cytometry. We found that the size distribution was multimodal. Smaller microbubbles were more abundant. Differential centrifugation was used to successfully isolate the 1-2 and 4-5 mu m diameter fractions. Isolated microbubbles were stable over two days. After two weeks, however, more dilute suspensions (