Structural and Functional Vocal Fold Epithelial Integrity Following Injury
Structural and Functional Vocal Fold Epithelial Integrity Following Injury
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DOI：
10.1002/lary.24818
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发表时间：
2014-12-01
期刊：
影响因子：
2.6
通讯作者：
Fried, Marvin P.
中科院分区：
文献类型：
--
作者：
Leydon, Ciara;Imaizumi, Mitsuyoshi;Fried, Marvin P.
目标/假设完整的上皮是声带防御的重要组成部分。上皮损伤会损害声带稳态并保护宿主组织免受病毒和细菌的侵袭。阐明损伤对上皮结构和屏障完整性的影响可以深入了解上皮在保护声带方面的作用。使用动物模型，我们评估了损伤后结构和功能上皮恢复的时间过程。研究设计前瞻性、对照动物研究。方法对40只大鼠进行单侧声带粘膜切除手术。损伤后 3 至 90 天之间每隔 5 个时间间隔采集喉部，并准备用于组织学和渗透性分析。 结果损伤后 5 天多层上皮、细胞间连接和基底膜的恢复表明结构完整性的快速恢复。损伤后5周内观察到非典型上皮通透性。结论上皮屏障完整性的恢复滞后于上皮结构的恢复。因此，上皮再生不能等同于功能屏障完整性的恢复。相反，再生上皮的持续渗漏表明声带仍然面临损伤、病原体入侵和损伤后重塑的风险。证据水平N/A。喉镜，124：2764-2769，2014
Objectives/HypothesisAn intact epithelium is an important part of vocal fold defense. Damage to the epithelium can compromise vocal fold homeostasis and protection of the host tissue from viral and bacterial invasion. Elucidating the effects of damage on epithelial architectural and barrier integrity provides insight into the role of epithelium in protecting vocal folds. Using an animal model, we evaluated the time course of structural and functional epithelial restoration following injury.Study DesignProspective, controlled animal study.MethodsForty rats underwent surgery to remove vocal fold mucosa unilaterally. Larynges were harvested at five time intervals between 3 to 90 days postinjury and were prepared for histological and permeability analyses.ResultsRapid restoration of structural integrity was demonstrated by return of a multilayerd epithelium, intercellular junctions, and basement membrane at 5 days postinjury. Atypical epithelial permeability was observed up to 5 weeks postinjury.ConclusionRestoration of epithelial barrier integrity lags epithelial structural restoration. Consequently, epithelial regeneration cannot be equated with return of functional barrier integrity. Rather, ongoing leakiness of regenerated epithelium indicates that vocal folds remain at risk for damage, pathogen invasion, and remodeling postinjury.Level of EvidenceN/A. Laryngoscope, 124:2764-2769, 2014