FINE STRUCTURE AND ONTOGENY OF TRICHOCYSTS IN MARINE DINOFLAGELLATES
FINE STRUCTURE AND ONTOGENY OF TRICHOCYSTS IN MARINE DINOFLAGELLATES
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DOI：
10.1007/bf01247920
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发表时间：
1966-01-01
期刊：
影响因子：
2.9
通讯作者：
SWEENEY, BM
中科院分区：
文献类型：
--
作者：
BOUCK, GB;SWEENEY, BM
在本报告中，在几种海洋甲藻的培养基中发现了长的，刚性的，杆状的结构，具有突出的棘孢囊的细横带特征。这些结构在属，如膝沟藻被认为是通过狭窄的孔隙通过重镀表面。在静止或“带电”状态下，发现包囊有一个精心制作的晶体核心，由一系列纤维和更细的原纤维连接到封闭囊的顶端。囊壁由单层膜和细线状环或螺旋组成。整个荷电包囊的设计暗示了一种机械传感装置。毛孢囊被发现起源于膜限制的囊泡，囊泡位于由高尔基体组成的球形壳内。最初，这些囊泡包含均匀的材料，但随着不断发展，出现晶格，最终演变为静止的囊泡核心。此时，胞囊离开高尔基体，迁移到细胞质的其他地方。发现带电的囊泡核心是水溶性的，但不溶于丙酮，相反，放电的囊泡不受任何溶剂的影响。这些事实与轴的发现显然是从无定形内容聚合被解释为支持水化理论的水化囊放电。最后，突出的囊肿轴和胶原纤维的起源和结构之间的惊人的相似之处进行了简要讨论。
Long, rigid, rod-like structures found in the culture medium of several marine dinoflagellates are shown in this report to have fine transverse bandings characteristic of extruded trichocysts. These structures in genera such as Gonyaulax are believed to pass through the heavily plated surface via narrow pores. In the resting or "charged" form, trichocysts are found to have an elaborate crystalline core connected by a series of fibers and still finer fibrils to the apex of an enclosing sac. The walls of this sac consist of a single membrane and fine thread-like hoops or spirals. The design of the whole charged trichocysts is suggestive of a mechanical sensing device. Trichocysts are found to originate in membrane-limited vesicles which are localized within a spherical shell composed of Golgi bodies. Initially these vesicles contain homogeneous materials, but with increasing development a crystal lattice appears and ultimately the resting trichocyst core evolves. At this point the trichocyst leaves the Golgi area and migrates elsewhere in the cytoplasm. The charged trichocyst core is found to be water- but not acetone-soluble in contrast to the discharged trichocyst which is unaffected by either solvent. These facts together with the finding of shafts apparently polymerizing from amorphous contents are interpreted as supporting the hydration theory of trichocyst discharge. Finally, the striking similarities between the origin and structure of extruded trichocyst shafts and the origin and structure of collagen fibers are discussed briefly.