A SIMPLE PROCEDURE FOR THE ISOLATION OF BRAIN ACTIN
A SIMPLE PROCEDURE FOR THE ISOLATION OF BRAIN ACTIN
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DOI：
10.1080/00327488108061774
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发表时间：
1981-01-01
期刊：
影响因子：
--
通讯作者：
HIMES, RH
中科院分区：
文献类型：
--
作者：
RUSCHA, MF;HIMES, RH
通过丙酮粉末形成含肌动蛋白凝胶，从牛脑中分离出肌动蛋白。凝胶增溶和层析在Sephadex G150上产生的肌动蛋白产率很高。脑匀浆粗粒的丙酮粉在分离微管蛋白中产生。从全脑丙酮粉中分离出80%的肌动蛋白。这种方法可以从同一个大脑中分离出这两种蛋白质。
Actin was isolated from bovine brain by forming an actin-containing gel from acetone powders. Solubilization of the gel and chromatography on Sephadex G150 produces actin in good yield. Acetone powders of crude pellets of brain homogenates obtained in the isolation of tubulin produces .apprx. 80% of the actin isolated from acetone powder of whole brain. The procedure can be used to isolate both proteins from the same brain.