Genetic Analysis of a QTL qPH3 for Plant Height in Rice

Genetic Analysis of a QTL qPH3 for Plant Height in Rice
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发表时间:
2012
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通讯作者:
Hu Pei-song
Hu Pei-song
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作者:
Hu Pei-song

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以粳稻(D50)与籼稻(HB277)杂交的重组自交系群体为材料,研究了影响株高的遗传因素。共鉴定出6个对株高具有显著加性效应的数量性状位点,分别位于第1、2、3、4、6和8号染色体上。其中1个QTL qPH3在浙江杭州和海南临水分别稳定表达,解释方差为16.52%和16.60%,具有显著的加性效应。从残杂合株系中选择1个残杂合株系(RHL),构建残杂合株系f2群体。在RHL-F2群体中检测到主QTL qPH3,解释方差为76.13%。通过子代测试,qPH3最终定位在RM16211-RM16237区域约204 kb内。这些结果将有助于该QTL的精细定位和基于图谱的克隆。
A recombinant inbred population derived from the cross between a javonica(D50)and an indica(HB277)rice was used to investigate the genetic factors affecting plant height.A total of six quantitative trait loci(QTLs)with significant additive effect were identified for the plant height,and they were located on chromosome 1,2,3,4,6 and 8.One QTL qPH3 can stably express and have significant additive effects in Hangzhou,Zhejiang Province with the explained variance of 16.52% and Linshui,Hainan Province with the explained variance of 16.60%.One residual heterozygous line(RHL) was selected from the RILs and a RHL-F2 population was constructed.The major QTL qPH3 with the explained variance of 76.13% was detected with the RHL-F2 population.By progeny tests,qPH3 was finally mapped within about 204 kb in the region RM16211-RM16237.These results will be helpful for fine mapping and map-based cloning of this QTL.