Prevalence and relationship of human papilloma virus type 16 and type 18 with oral squamous cell carcinoma and oral leukoplakia in fresh scrappings: a PCR study.
Prevalence and relationship of human papilloma virus type 16 and type 18 with oral squamous cell carcinoma and oral leukoplakia in fresh scrappings: a PCR study.
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新鲜碎屑中人乳头瘤病毒 16 型和 18 型与口腔鳞状细胞癌和口腔白斑的患病率及其关系：PCR 研究。
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发表时间：
2011
期刊：
影响因子：
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通讯作者：
Kedar Saraf
中科院分区：
文献类型：
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作者：
Asok Mathew;R. N. Mody;Mahendra Patait;Ali A. Razooki;N. T. Varghese;Kedar Saraf
引言
当你研究恶性肿瘤及其发病机制时，它一直是一个弥漫清晰的区域。近年来，病毒在致癌过程中的作用引起了研究人员的极大兴趣。分子生物学和生物技术领域的最新进展已经解决了一些关于发病机制的问题。人乳头瘤病毒(HPV)及其在宫颈恶性肿瘤发生中的作用已被证明几乎是毋庸置疑的。
目标
本研究旨在探讨HPV16和18型在口腔癌前病变和鳞癌发生中的作用。本研究还旨在利用聚合酶链式反应(PCR)来了解组织学诊断为口腔白斑和口腔鳞状细胞癌的这些类型的患病率及其与吸烟习惯的相关性。
材料和方法
本研究选择了45例经组织病理证实的口腔鳞状细胞癌患者，年龄在32~85岁，20例口腔白斑，年龄在22~66岁。所有样本均进行聚合酶链式反应。聚合酶链式反应在PTC 200热循环仪[MJ Research Inc.，沃特敦，美国马萨诸塞州]中进行。
结果
使用从新鲜刮片中获得的非常简单的非侵入性刮片来分析这两种病毒的现场流行率和混合感染率，发现它们确实很高。口腔鳞癌患者HPV16型阳性率为73.3%(33/45)，HPV18型阳性率为71.1%(32/45)，HPV16型和18型均阳性者占57.7%(26/45)。
结论
HPV16型、18型及其混合感染在口腔鳞状细胞癌中的患病率较高，在口腔白斑中的患病率高于HPV16型及混合感染。观察到口腔鳞状细胞癌患者的舌和腭部病变与其他部位相比，HPV16型、18型和混合感染的发生率较高。
INTRODUCTION
It has been always an area of diffuse clarity when you study malignancy and its pathogenesis. Recently, it has invited lot of interest among the researchers about the possibility of role of viruses in the initiation of carcinogenesis. Recent advances in the field of molecular biology and biotechnology have solved some problems with regard to pathogenesis. Human papilloma virus (HPV) and its role in the initiation of malignancy in the cervix is proven almost beyond doubt.
OBJECTIVES
The present study is aimed at the role of two types of HPV 16 and 18 in the initiation of oral premalignant and squamous cell carcinoma. The study also aims at using polymerase chain reaction (PCR) in finding out the prevalence of these types diagnosed histologically as oral leukoplakia and oral squamous cell carcinoma and prevalence of its association with the habit of tobacco use.
MATERIALS AND METHODS
In the present study, 45 patients having histopathologically confirmed oral squamous cell carcinoma in the age range of 32-85 years were selected along with 20 histopathologically confirmed oral leukoplakia in the age range 22-66 years. All the samples were subjected to polymerase chain reaction. The PCR reaction was carried out in PTC 200 thermo-cycler [MJ Research Inc, Watertown, MA, USA].
RESULTS
The site prevalence and co-infection rate of these two types of viruses are being analyzed using very simple non-invasive scrapings obtained from fresh scrapings and found to be really high. It was also observed that 73.3% (33/45) of the oral squamous cell carcinoma patients were positive for oral HPV type 16 while 71.1% (32/45) were positive for HPV type 18 infection and 57.7% (26/45) were found to have both HPV type 16 and HPV type 18 infections.
CONCLUSIONS
HPV type 16, 18, and co-infection of both types showed high prevalence in oral squamous cell carcinoma.The prevalence of HPV type 18 was found to be higher than HPV type 16 and co-infection in oral leukoplakia. It was observed that the tongue and palate lesions in the oral squamous cell carcinoma patients showed high prevalence of HPV type 16, type 18, and co-infection compared with other sites.