The photo-thermochemical properties and functions of Marchantia phototropin encoded by an unduplicated gene in land plant evolution
The photo-thermochemical properties and functions of Marchantia phototropin encoded by an unduplicated gene in land plant evolution
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陆地植物进化中不重复基因编码的地钱向光素的光热化学性质和功能
DOI：
10.1016/j.jphotobiol.2021.112305
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发表时间：
2021
期刊：
影响因子：
--
通讯作者：
Kodama Yutaka
中科院分区：
文献类型：
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作者：
Kato Shota;Takahashi Yamato;Fujii Yuta;Sasaki Kotoko;Hirano Satoyuki;Okajima Koji;Kodama Yutaka
趋光素（Phototropin，phot）是植物中的蓝光感受器，具有两个光氧电压（LOV 1和LOV 2）结构域，具有不同的光热化学性质。而地钱含有PHOT的单个拷贝（例如，MpPHOTinMarchantia polymorpha），许多陆地植物物种含有多个PHOTgenes（例如，AtPHOT 1和2在拟南芥）由于进化的基因重复。复制的phot蛋白的LOV结构域已被详细研究，但单拷贝的phot蛋白的LOV结构域仍有待表征。由于phot在地钱中没有复制，我们假设Mpphot可能保留了祖先的功能和光热化学性质。为了了解更多关于非重复的phot蛋白，我们分析了叶绿体移位运动和光-热化学性质的LOV 1和LOV 2在Mpphot（Mpphot-LOV 1和Mpphot-LOV 2，分别）。Mpphot-LOV 1的功能与A. Thaliana（例如，Atphot 1-LOV 1阻止蓄积反应）。另一方面，Mpphot-LOV 2的功能与复制光的LOV 2相似。Mpphot的光-热化学性质是那些复制的PHOTS的混合物; Mpphot的光化学和热化学反应分别类似于PHOT 2-和PHOT 1-型蛋白。我们的研究结果揭示了在陆地植物进化过程中，LOV结构域之间的保守性和多样性。
Phototropin (phot) is a blue light photoreceptor in plants and possesses two photosensory light‑oxygen-voltage (LOV1 and LOV2) domains with different photo-thermochemical properties. While liverworts contain a single copy ofPHOT(e.g.,MpPHOTinMarchantia polymorpha), many land plant species contain multicopyPHOTgenes (e.g.,AtPHOT1and2inArabidopsis thaliana) due to evolutionary gene duplication. The LOV domains of duplicated phot proteins have been studied in detail, but those of single-copy phot proteins remain to be characterized. As phot has not been duplicated in liverworts, we hypothesized that Mpphot may retain the ancestral function and photo-thermochemical properties. To learn more about the unduplicated phot proteins, we analyzed chloroplast relocation movement and the photo-thermochemical properties of LOV1 and LOV2 in Mpphot (Mpphot-LOV1 and Mpphot-LOV2, respectively). The function of Mpphot-LOV1, which induced a response to move chloroplasts to weak light (the accumulation response) in the absence of photoactive LOV2, differed from that of LOV1 of the duplicated phot proteins ofA. thaliana(e.g., Atphot1-LOV1 preventing the accumulation response). On the other hand, the function of Mpphot-LOV2 was similar to that of LOV2 of the duplicated phots. The photo-thermochemical properties of Mpphot were a hybrid of those of the duplicated phots; the photochemical and thermochemical reactions of Mpphot were similar to those of the phot2- and phot1-type proteins, respectively. Our findings reveal conservation and diversification among LOV domains during phot duplication events in land plant evolution.