A NOVEL FORM OF EPSTEIN-BARR-VIRUS LATENCY IN NORMAL B-CELLS IN-VIVO
A NOVEL FORM OF EPSTEIN-BARR-VIRUS LATENCY IN NORMAL B-CELLS IN-VIVO
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DOI：
10.1016/0092-8674(95)90513-8
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发表时间：
1995-02-24
期刊：
影响因子：
64.5
通讯作者：
THORLEYLAWSON, DA
中科院分区：
文献类型：
--
作者：
MIYASHITA, EM;YANG, B;THORLEYLAWSON, DA
我们已经开发了一种PCR检测方法，可以在10（6）个未感染细胞的存在下检测单个EB病毒（EBV）基因组。使用该检测方法，我们证明了EBV在所有检测的血清阳性个体的外周血中，在CD 19（+）、CD 23（-）和CD 80（B7）（-）B细胞中持续存在。我们进一步表明，这些细胞中的病毒是潜伏的，但很容易重新激活，产生感染性永生化病毒，因此，这些细胞代表了一个真正的网站的潜伏持久性。EBV在单核细胞和T细胞中均未检出。在9名健康供体中，感染细胞的频率从每10（7）个B细胞23个到625个不等，但在2年的过程中，每个个体的感染细胞的频率相对稳定。我们的结论是，体内EBV感染的细胞是B细胞与非活化表型。这代表了正常B细胞中一种新的潜伏形式。
We have developed a PCR assay that can detect a single Epstein-Barr virus (EBV) genome in the presence of 10(6) uninfected cells. Using this assay, we demonstrate that EBV persists, in the peripheral blood of all seropositive individuals tested, in CD19(+), CD23(-), and CD80 (B7)(-) B cells. We further show that the virus in these cells is latent, but readily reactivated to produce infectious immortalizing virus; therefore, these cells represent a true site of latent persistence. EBV was not significantly detected in monocytes or T cells. The frequency of infected cells in nine healthy donors varied from 23 to 625 per 10(7) B cells, but was relatively stable for each individual over the course of 2 years. We conclude that the EBV-infected cells in vivo are B cells with a nonactivated phenotype. This represents a novel form of latency in normal B cells.