A novel biomimetic olfactory-based biosensor for single olfactory sensory neuron monitoring.
A novel biomimetic olfactory-based biosensor for single olfactory sensory neuron monitoring.
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DOI：
10.1016/j.bios.2008.07.065
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发表时间：
2009
影响因子：
12.6
通讯作者：
Chunsheng Wu;Peihua Chen;Hui Yu;Qingjun Liu;Xiaolin Zong;H. Cai;Ping Wang
中科院分区：
文献类型：
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作者：
Chunsheng Wu;Peihua Chen;Hui Yu;Qingjun Liu;Xiaolin Zong;H. Cai;Ping Wang
基于光寻址电位传感器（LAPS），以大鼠嗅感觉神经元（OSNs）为敏感元件，构建了一种新型仿生嗅觉生物传感器，用于嗅觉信号转导机制的研究。大鼠OSN在LAPS芯片表面培养。为了验证LAPS记录的电信号的来源，测试了MDL 12330 A对OSN的嗅觉信号的抑制作用，MDL 12330 A是腺苷酸环化酶的特异性抑制剂。研究了磷脂酰肌醇3-激酶（PI 3 K）特异性抑制剂LY 294002对OSNs的增强作用。结果表明，该复合生物传感器能够以非侵入的方式长期有效地记录OSNs对气味的响应，MDL 12330 A对OSNs的响应具有抑制作用，LY 294002对OSNs的响应具有增强作用。所有这些结果表明，这种混合生物传感器可以用于监测OSNs的电生理在一个非侵入性的方式，并建议它可能是一个有前途的工具，研究嗅觉转导机制。
This paper presents a novel biomimetic olfactory biosensor for the study of olfactory transduction mechanisms on the basis of light addressable potentiometric sensor (LAPS), in which rat olfactory sensory neurons (OSNs) are used as sensing elements. Rat OSNs are cultured on the surface of LAPS chip. To validate the origin of the electrical signals recorded by LAPS, the inhibitory effect of MDL12330A to the olfactory signals of OSNs is tested, which is the specific inhibitor of adenylyl cyclase. The enhancive effect of LY294002 to the responses of OSNs is also investigated, which is the specific inhibitor of phosphatidylinositol 3-kinase (PI3K). The results show that this hybrid biosensor can record the responses of OSNs to odours efficiently in a non-invasive way for a long term, and the responses can be inhibited by MDL12330A and enhanced by LY294002. All these results demonstrate that this hybrid biosensor can be used to monitor electrophysiology of OSNs in a non-invasive way and suggest it could be a promising tool for the study of olfactory transduction mechanisms.