A simple HPLC method for the determination of bifendate: Application to a pharmacokinetic study of bifendate liposonie
A simple HPLC method for the determination of bifendate: Application to a pharmacokinetic study of bifendate liposonie
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DOI：
10.1016/j.jchromb.2007.07.040
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发表时间：
2007-10-01
影响因子：
3
通讯作者：
Xiao, Yan-Yu
中科院分区：
文献类型：
--
作者：
Chen, Zhi-Peng;Zhu, Jia-Bi;Xiao, Yan-Yu
建立了一种快速、灵敏、简便的紫外检测器高效液相色谱法测定100 μ l大鼠血浆中联苯双酯的方法。用100 μ l乙腈脱蛋白制备样品。以甲醇-双蒸馏水（65/35,v/v）为流动相，流速为1.0 ml/min，将上清液20 μ l直接注入高效液相色谱系统。色谱柱为Bondapak C-18，温度为30℃，峰位于278 nm处。血药浓度在0.028 ~ 2.80 μ g/ml范围内，线性关系良好（r(2) = 0.9989）。日内和日间变异系数分别不大于6.55%和6.07%。检出限为5 ng/ml。联苯酯在血浆中的平均加标回收率为94.53% ~ 99.36%。本方法已成功应用于联苯酯脂质体在大鼠体内的药动学研究。(c) 2007 Elsevier B.V.版权所有
A rapid, sensitive and simple high-performance liquid chromatographic (HPLC) method with ultraviolet detector (UV) has been developed for the determination of bifendate in 100 mu l plasma of rats. Sample preparation was carried out by deproteinization with 100 mu l of acetonitrile. A 20 mu l of supernatant was directly injected into the HPLC system with methanol-double distilled water (65/35, v/v) as the mobile phase at a flow rate of 1.0 ml/min. Separation was performed with a mu Bondapak C-18 column at 30 degrees C. The peak was detected at 278 nm. The calibration curve was linear (r(2) = 0.9989) in the concentration range of 0.028-2.80 mu g/ml in plasma. The intra- and inter-day variation coefficients were not more than 6.55% and 6.07%, respectively. The limit of detection was 5 ng/ml. The mean recoveries of bifendate were ranged from 94.53% to 99.36% in plasma. The present method has been successfully applied to the pharmacokinetic study of bifendate liposome in rats. (c) 2007 Elsevier B.V. All rights reserved.