Genome-wide identification of QTL conferring high-temperature adult-plant (HTAP) resistance to stripe rust (Puccinia striiformis f. sp tritici) in wheat
Genome-wide identification of QTL conferring high-temperature adult-plant (HTAP) resistance to stripe rust (Puccinia striiformis f. sp tritici) in wheat
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DOI：
10.1007/s11032-011-9590-x
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发表时间：
2012-03-01
影响因子：
3.1
通讯作者：
Zemetra, Robert
中科院分区：
文献类型：
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作者：
Chen, Jianli;Chu, Chenggen;Zemetra, Robert
高温成株（HTAP）对条锈病（由小麦条锈菌专化型（Puccinia striiformis f. sp. tritici）引起）的抗性是小麦（Triticum aestivum L.）的持久抗性类型。本研究在由 169 个 F-8:10 重组自交系 (RIL) 组成的群体中鉴定了赋予 HTAP 对条锈病抗性的数量性状位点 (QTL)，这些自交系源自易感品种 Rio Blanco 和抗性种质 IDO444 之间的杂交。在两年的时间里，在两个地点对自然感染下的疾病严重程度和感染类型进行了 HTAP 抗性评估。遗传连锁图谱在整个基因组中每个标记的平均密度为 6.7 cM，并使用 484 个标记构建，包括 96 个小麦微卫星 (SSR)、632 个多样性阵列技术 (DArT) 多态性、来自半矮秆基因 Rht1 和 Rht2 的两个序列标记位点 (STS) 以及两个低分子量麦谷蛋白基因亚基标记。 QTL分析共检测到8个与HTAP抗条锈病显着相关的QTL，其中2B染色体上2个，3B染色体上2个，1A、4A、4B和5B染色体上各1个。 2B 和 4A 号染色体上的 QTL 是源自 IDO444 的主要基因座，分别解释了疾病严重程度和感染类型的表型变异的 47% 和 42%。其余5个QTL占性状变异的7-10%。在这些次要 QTL 中，两个 QTL QYrrb.ui-3B.1 和 QYrrb.ui-4B 的抗性等位基因源自 Rio Blanco，仅降低感染类型，而其他三个 QTL QYrid.ui-1A、QYrid.ui-3B.2 和 QYrid.ui-5B 的抗性等位基因均源自 IDO444，并降低感染类型或疾病严重程度。与 2B 和 4A QTL 相关的标记对于选择 HTAP 对条锈病的抗性应该是有用的。
High-temperature adult-plant (HTAP) resistance to stripe rust (caused by Puccinia striiformis f. sp. tritici) is a durable type of resistance in wheat (Triticum aestivum L.). This study identified quantitative trait loci (QTL) conferring HTAP resistance to stripe rust in a population consisting of 169 F-8:10 recombinant inbred lines (RILs) derived from a cross between a susceptible cultivar Rio Blanco and a resistant germplasm IDO444. HTAP resistance was evaluated for both disease severity and infection type under natural infection over two years at two locations. The genetic linkage maps had an average density of 6.7 cM per marker across the genome and were constructed using 484 markers including 96 wheat microsatellite (SSR), 632 Diversity Arrays Technology (DArT) polymorphisms, two sequence-tagged-site (STS) from semi-dwarf genes Rht1 and Rht2, and two markers for low molecular-weight glutenin gene subunits. QTL analysis detected a total of eight QTL significantly associated with HTAP resistance to stripe rust with two on chromosome 2B, two on 3B and one on each of 1A, 4A, 4B and 5B. QTL on chromosomes 2B and 4A were the major loci derived from IDO444 and explained up to 47 and 42% of the phenotypic variation for disease severity and infection type, respectively. The remaining five QTL accounted for 7-10% of the trait variation. Of these minor QTL, the resistant alleles at the two QTL QYrrb.ui-3B.1 and QYrrb.ui-4B derived from Rio Blanco and reduced infection type only, while the resistant alleles at the other three QTL, QYrid.ui-1A, QYrid.ui-3B.2 and QYrid.ui-5B, all derived from IDO444 and reduced either infection type or disease severity. Markers linked to 2B and 4A QTL should be useful for selection of HTAP resistance to stripe rust.