Affinity Purification of IgG Subclasses and the Distribution of Thyroid Auto‐Antibody Reactivity in Hashimoto's Thyroiditis
Affinity Purification of IgG Subclasses and the Distribution of Thyroid Auto‐Antibody Reactivity in Hashimoto's Thyroiditis
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桥本甲状腺炎中 IgG 亚类的亲和纯化和甲状腺自身抗体反应性的分布
DOI：
10.1111/j.1365-3083.1989.tb01190.x
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发表时间：
1989
影响因子：
3.7
通讯作者：
C. B. Reimer
中科院分区：
文献类型：
--
作者：
A. Weetman;C. M. Black;S. Cohen;R. Tomlinson;J. Banga;C. B. Reimer
为准确描述甲状腺自身抗体的免疫球蛋白亚类分布，用完全去除亲和层析柱上其他免疫球蛋白亚类的方法，将9例桥本甲状腺炎患者血清分离为免疫球蛋白亚类。所有的免疫球蛋白亚类组分都含有甲状腺球蛋白和微粒体(或甲状腺过氧化物酶)抗体活性，尽管与血清中免疫球蛋白亚类的总浓度相比。IgG4抗体过度表达。然而，与最近的研究相比，这个特殊的亚类从未占主导地位-IgG4抗体水平被IgG1和IgG2亚类的抗体水平超过：这些差异似乎与以前使用的检测方法中不同亚类的不同灵敏度有关。这种亚类活性模式不同于在6名受试者中发现的破伤风类毒素抗体。在任何亚类中均未发现轻链限制，表明IgG4甲状腺抗体的过量表达不是由单抗引起的。两种甲状腺抗原亚类的功能亲和力因患者而异，但在大多数样本中，IgG2亚类部分显示出最高的功能亲和力。我们还发现，与其他三个亚类不同，IgG2亚类甲状腺抗体在诱导抗体依赖的细胞介导的细胞毒方面无效。我们的结果表明，甲状腺抗体在其免疫球蛋白亚类分布中受到的限制较少，患者的异质性也比先前描述的要少。此外，IgG2甲状腺抗体在数量上很重要，在相对功能亲和力和效应功能上不同于IgG1和IgG4甲状腺抗体。
To delineate accurately the IgG subclass distribution of thyroid auto‐antibodies, sera from nine patients with Hashimoto's thyroiditis were fractionated into IgG subclasses by complete depletion of the other IgG subclasses on affinity columns. All IgG subclass fractions contained thyroglobulin and microsomal (or thyroid peroxidase) antibody activity, although when compared to the total serum concentrations of IgG subclasses. IgG4 antibodies were over‐represented. However, in contrast to recent studies, this particular subclass never predominated—IgG4 antibody levels being exceeded by those of the IgG1 and IgG2 subclasses: it seems likely that these differences relate to varying sensitivity for different subclasses in previously used assay methods. This pattern of subclass activity differed from that of tetanus toxoid antibodies, which were found in six subjects. There was no light chain restriction within any subclass, showing that the overproduction of IgG4 thyroid antibodies is not of monoclonal origin. The functional affinity of subclasses for both thyroid antigens varied between patients, but IgG2 subclass fractions showed the highest functional affinity in the majority of samples. We also found that IgG2 subclass thyroid antibodies were ineffective in eliciting antibody‐dependent cell‐mediated cytotoxicity, as distinct from the other three subclasses. Our results show that thyroid antibodies are less restricted in their IgG subclass distribution and patients are less heterogeneous than previously described. Moreover, IgG2 thyroid antibodies are quantitatively important and differ in relative functional affinity and effector function from IgG1 and IgG4 thyroid antibodies.
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菌株依赖性 IgG 亚类反应模式。
DOI：
--
发表时间：
1987
期刊：
Journal of immunology (Baltimore, Md. : 1950)
影响因子：
--
作者：
Slack,JH
通讯作者：
Slack,JH
补体激活和抗甲状腺球蛋白 IgG 亚类限制的研究。
影响因子：
4.6
作者：
Adler,TR;Beall,GN;Curd,JG;Heiner,DC;Sabharwal,UK
通讯作者：
Sabharwal,UK
分离甲状腺微粒体抗原的互补 DNA 克隆。
DOI：
10.1172/jci113181
发表时间：
1987
期刊：
The Journal of clinical investigation
影响因子：
--
作者：
Seto,P;Hirayu,H;Magnusson,RP;Gestautas,J;Portmann,L;DeGroot,LJ;Rapoport,B
通讯作者：
Rapoport,B
人甲状腺自身抗体免疫球蛋白 G 亚类的受限异质性和 T 细胞依赖性。
DOI：
10.1210/jcem-62-5-945
发表时间：
1986
期刊：
The Journal of clinical endocrinology and metabolism
影响因子：
--
作者：
Davies,TF;Weber,CM;Wallack,P;Platzer,M
通讯作者：
Platzer,M