Cytokine expression in spleen affects progression of liver cirrhosis through liver-spleen cross-talk
Cytokine expression in spleen affects progression of liver cirrhosis through liver-spleen cross-talk
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DOI：
10.1111/hepr.12267
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发表时间：
2014-11-01
影响因子：
4.2
通讯作者：
Shimada, Mitsuo
中科院分区：
文献类型：
--
作者：
Asanoma, Michihito;Ikemoto, Tetsuya;Shimada, Mitsuo
目的脾脏是否通过肝脾相互作用影响肝硬化的进展尚不清楚。转化生长因子-1（Transforming growth factor-1，TGF-1）是目前报道的最强的肝纤维化细胞因子，白细胞介素-6（interleukin-6，IL-6）是肝再生的重要因子。在这项研究中，我们调查了细胞因子的表达，在脾脏的LC患者，以试图证明存在的肝，脾cross-talk.MethodsThe研究纳入了22例谁在我们的研究所进行脾切除术之间的2004年和2010年。采用免疫组化染色法检测脾组织中TGF-1的表达。采用免疫荧光法对脾脏中的CD 68和TGF-1进行双色染色以检测TGF-1的来源。结果LC患者脾组织中TGF-1的表达明显高于正常肝组织（P
AimIt is unclear whether the spleen affects the progression of liver cirrhosis (LC) through liver-spleen cross-talk. Transforming growth factor-1 (TGF-1) is reported to be the most potent cytokine of liver fibrosis, and interleukin-6 (IL-6) is an important factor of liver regeneration. In this study, we investigated the expression of cytokines in the spleens of LC patients in order to attempt to prove the existence of liver-spleen cross-talk.MethodsThe study enrolled 22 patients who underwent splenectomy at our institute between 2004 and 2010. TGF-1 expression in the resected spleen was measured using immunohistochemical staining. Two-color immunofluorescent staining for CD68 and TGF-1 in the spleen was performed to detect sources of TGF-1. IL-6 expression in the spleen was measured by reverse transcription polymerase chain reaction.ResultsTGF-1 expression was significantly higher in the spleens of LC patients than in those of patients with normal livers (P