The involvement of cell wall expansion in the two modes of mycelium formation of Candida albicans.
The involvement of cell wall expansion in the two modes of mycelium formation of Candida albicans.
复制标题
细胞壁扩张参与白色念珠菌菌丝形成的两种模式。
DOI：
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发表时间：
1985
期刊：
影响因子：
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通讯作者：
M. Staebell
中科院分区：
文献类型：
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作者：
D. Soll;M. Herman;M. Staebell
当白色念珠菌的芽殖细胞饥饿20分钟，然后稀释到37 ℃，pH 6.7的新鲜营养培养基中时，它们通过两种交替模式形成菌丝体。对于具有小芽的细胞，芽向顶端扩展，导致瞬时锥形的子细胞。随着继续生长，子细胞逐渐变细成为细长的菌丝体。对于具有大芽的细胞，芽以出芽形式完成扩展，母细胞然后子芽外翻，并且外翻生长为菌丝体。本研究调查是否在时间和空间上的变化，在区域的扩展在芽生长菌丝体形成的两种模式参与。数据表明，决定菌丝体形成的两种模式的过渡周长和活性顶端扩张区关闭的过渡周长均为7微米。这种确切的相关性表明，由于仍然存在的顶端扩展区的再激活，具有周长小于7微米的芽的饥饿细胞以锥形模式形成菌丝体，并且由于在饥饿时不存在顶端扩展区，具有周长大于7微米的芽的饥饿细胞通过一般扩展完成芽生长。
When budding cells of Candida albicans are starved for 20 min and then diluted into fresh nutrient medium at 37 degrees C, pH 6.7, they form mycelia by two alternative modes. For cells with small buds, the bud expands apically, resulting in a transiently tapered daughter cell. With continued growth, the daughter cell tapers into an elongated mycelium. For cells with large buds, the bud completes expansion in the budding form, the mother cell and then the daughter bud evaginate, and the evaginations grow as mycelia. The present study investigates whether the temporal and spatial changes in the zones of wall expansion during bud growth are involved in the two modes of mycelium formation. Data are presented which demonstrate that the transition circumference which determines the two modes of mycelium formation and the transition circumference at which the active apical expansion zone shuts down are both 7 micron. This exact correlation suggests that starved cells with buds with a circumference of less than 7 micron form mycelia in the tapering mode due to the reactivation of the still present apical expansion zone, and that starved cells with buds with a circumference greater than 7 micron complete bud growth by general expansion due to the absence of the apical expansion zone at the time of starvation.