Cloning and characterization of a class II DNA photolyase from Chlamydomonas
Cloning and characterization of a class II DNA photolyase from Chlamydomonas
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DOI：
10.1023/a:1006279720960
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发表时间：
1999-08
影响因子：
5.1
通讯作者：
J. L. Petersen;D. W. Lang;G. Small
中科院分区：
文献类型：
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作者：
J. L. Petersen;D. W. Lang;G. Small
由紫外线引起的DNA损伤可以通过一种由DNA光解酶催化的蓝光依赖性反应来逆转。衣原体在细胞核和叶绿体中都具有DNA光裂合酶活性。在这里，我们报告的克隆和测序的基因，PHR2，从衣原体编码II类DNA光裂合酶。PHR2蛋白，当在大肠杆菌中表达时，能够补充DNA光裂合酶缺陷。先前描述的衣原体突变体，phr1，这是在核缺陷，但不是叶绿体光裂合酶活性的RFLP分析表明，不与PHR2基因。与最近报道的拟南芥II类DNA光解酶不同，PHR2编码的蛋白质被预测含有叶绿体靶向序列。这一结果与RFLP数据一起表明PHR2编码叶绿体靶向DNA光解酶。
Damage to DNA induced by ultraviolet light can be reversed by a blue light-dependent reaction catalyzed by enzymes called DNA photolyases. Chlamydomonas has been shown to have DNA photolyase activity in both the nucleus and the chloroplast. Here we report the cloning and sequencing of a gene, PHR2, from Chlamydomonas encoding a class II DNA photolyase. The PHR2 protein, when expressed in Escherichia coli, is able to complement a DNA photolyase deficiency. The previously described Chlamydomonas mutant, phr1, which is deficient in nuclear but not chloroplast photolyase activity was shown by RFLP analysis not to be linked to the PHR2 gene. Unlike the recently reported class II DNA photolyase from Arabidopsis, the protein encoded by PHR2 is predicted to contain a chloroplast targeting sequence. This result, together with the RFLP data, suggests that PHR2 encodes the chloroplast targeted DNA photolyase.