Formation of lipid droplets induced by 2,3-dihydrogeranylgeranoic acid distinct from geranylgeranoic acid
Formation of lipid droplets induced by 2,3-dihydrogeranylgeranoic acid distinct from geranylgeranoic acid
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DOI：
10.18388/abp.2007_3158
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发表时间：
2007-01-01
影响因子：
1.7
通讯作者：
Sagami, Hiroshi
中科院分区：
文献类型：
--
作者：
Kodaira, Yuichi;Kusumoto, Takeru;Sagami, Hiroshi
香叶基香叶酸（GGA）和2，3-二氢香叶基香叶酸（2，3-diGGA）是香叶基香叶醇衍生的代谢物（科代拉等人（2002）J Biochem 132：327-334）。在本研究中，我们研究了这些酸对HL-60细胞的作用。通过类似于视黄酸刺激的GGA刺激，细胞分化为中性粒细胞。在用2，3-diGGA刺激的细胞的情况下，未检测到嗜中性粒细胞，但诱导了脂滴的形成。另一方面，当细胞在0.1%FBS而不是10%FBS的存在下培养时，不仅通过GGA刺激，而且通过2，3-diGGA也诱导凋亡细胞。在后一种情况下，当在半胱天冬酶-3抑制剂（Ac-DMQD-CHO）的共同存在下培养细胞时，在细胞中观察到脂滴形成。这些结果表明，GGA和2，3-diGGA在它们对HL-60细胞的作用方面彼此极其不同。
Geranylgeranoic acid (GGA) and 2,3-dihydrogeranylgeranoic acid (2,3-diGGA) are geranylgeraniol-derived metabolites (Kodaira et al. (2002) J Biochem 132: 327-334). In the present study, we examined the effects of these acids on HL-60 cells. The cells were differentiated into neutrophils by GGA stimulation like retinoic acid stimulation. In the case of cells stimulated with 2,3-diGGA, neutrophils were not detected, but the formation of lipid droplets was induced. On the other hand, when the cells were cultured in the presence of 0.1% FBS instead of 10% FBS, apoptotic cells were induced not only by GGA stimulation but also with 2,3-diGGA. In the latter case, when the cells were cultured in the co-presence of a caspase-3 inhibitor (Ac-DMQD-CHO), the lipid droplets formation was observed in the cells. These results suggest that GGA and 2,3-diGGA are extremely different from each other with respect to their effects on HL-60 cells.