Phenotypic and genotypic characterization of PVL-positive Staphylococcus aureus isolated from retail foods in China
Phenotypic and genotypic characterization of PVL-positive Staphylococcus aureus isolated from retail foods in China
复制标题
中国零售食品中分离出的 PVL 阳性金黄色葡萄球菌的表型和基因型特征
DOI：
10.1016/j.ijfoodmicro.2019.05.021
复制
发表时间：
2019
影响因子：
5.4
通讯作者：
Ding Yu
中科院分区：
文献类型：
--
作者：
Wu Shi;Zhang Feng;Huang Jiahui;Wu Qingping;Zhang Jumei;Dai Jingsha;Zeng Haiyan;Yang Xiaojuan;Chen Moutong;Pang Rui;Lei Tao;Zhang Youxiong;Xue Liang;Wang Juan;Ding Yu
金黄色葡萄球菌编码许多已知或强烈怀疑会导致特定疾病或症状的毒素。潘顿-瓦伦丁杀白素(PVL)是这些与高死亡率相关的重要毒素之一。在我们之前的研究中，1581S。2011年至2016年，从中国大部分省会城市的4,300份零售食品样品中分离出金黄色葡萄球菌。本研究旨在调查PVL阳性患者的患病率。从中国零售食品中分离出金黄色葡萄球菌，并对其进行耐药性检测、分型、多位点分型和肠毒素基因分析。总共有72株(4.6%)菌株含有pv1基因，其中24.1%的MRSA菌株(26/108)和3.1%的MSSA菌株(46/1473)含有pv1基因，涉及不同种类的食品。共分出17个序列类型(STS)和27个spat型，其中pVL阳性占43.1%(31/72)。金黄色葡萄球菌属于CC59-T437。这些分离株至少含有sei(97.2%)、sem(86.1%)、seq(80.6%)、seg(68.1%)、sek(68.1%)、seb(62.5%)、sel(52.8%)、sej(50.0%)、seh(48.6%)、sep(45.8%)、sep(38.9%)、ser(37.5%)、sen(27.8%)、sec(16.7%)、see(16.7%)、SED(6.9%)、SEO(6.9%)和SEU(6.7%)。占总数的87.5%。金黄色葡萄球菌(63/72)含有典型的SE基因(SEA、SEB、SEC、SED和SEE)，而所有的SE基因。金黄色葡萄球菌含有egc簇基因(seg、sei、sem、sen、seo和seu)。在药敏试验中，98.6%(71/72)的菌株至少对一种抗生素耐药，其中47株为多重耐药。对青霉素(94.4%)、红霉素(83.4%)、克林霉素(63.9%)、卡那霉素(61.1%)、泰红霉素(58.3%)、链霉素(51.4%)、四环素(47.2%)、氯霉素(27.8%)、呋西地酸(27.8%)及其他抗生素耐药率为20%。所有分离的革兰氏阴性杆菌均为CC59-T437，为中国地区社区相关(CA)-MRSA的优势菌型。这些分离物在食品中的存在对消费者构成潜在的健康风险，需要进一步关注。
Staphylococcus aureus encodes numerous toxins that are known or strongly suspected to cause specific diseases or symptoms. Panton-Valentine leukocidin (PVL) is one of these important toxins that is associated with high mortality rates. In our previous study, 1581S. aureusstrains were isolated from 4300 samples of retail foods obtained from most of the provincial capitals in China from 2011 to 2016. This study aimed to investigate the prevalence of PVL-positiveS. aureusisolates from retail foods in China and characterize these isolates by antibiotic resistance testing,spatyping, multilocus sequence typing (MLST) and enterotoxin gene analyses. In total, seventy-two isolates (72/1581, 4.6%) possessedpvlgenes, including 24.1% MRSA isolates (26/108) and 3.1% MSSA isolates (46/1473), covering different types of food. The strains were divided into seventeen sequence types (STs) and twenty-sevenspatypes, and 43.1% (31/72) of the PVL-positiveS. aureusisolates belonged to CC59-t437. These isolates contained at least one of the following enterotoxin genes:sei(97.2%),sem(86.1%),seq(80.6%),seg(68.1%),sek(68.1%),seb(62.5%),sel(52.8%),sej(50.0%),seh(48.6%),sep(45.8%),sea(38.9%),ser(37.5%),sen(27.8%),sec(16.7%),see(16.7%),sed(6.9%),seo(6.9%) andseu(6.7%). A total of 87.5% of theS. aureusisolates (63/72) harboured the classic SE genes (sea,seb,sec,sed, andsee), whereas all theS. aureusisolates harboured the genes of theegccluster (seg,sei,sem,sen,seo, andseu). In antimicrobial susceptibility tests, 98.6% of the isolates (71/72) exhibited resistance to at least one antibiotic, including 47 multi-drug-resistant isolates. Resistance to penicillin (94.4%), erythromycin (83.4%), clindamycin (63.9%), kanamycin (61.1%), telithromycin (58.3%), streptomycin (51.4%), tetracycline (47.2%), chloramphenicol (27.8%), fusidic acid (27.8%) and other antibiotics (<20%) was observed. All the PVL-positive MRSA isolates belonged to CC59-t437, which is the predominant type of community-associated (CA)-MRSA in China. The presence of these isolates in food represents a potential health risk for consumers and warrants further attention.