Iron plays a key role in the cytodifferentiation of human periodontal ligament cells
Iron plays a key role in the cytodifferentiation of human periodontal ligament cells
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DOI：
10.1111/jre.12103
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发表时间：
2014-04-01
影响因子：
3.5
通讯作者：
Murakami, S.
中科院分区：
文献类型：
--
作者：
Hou, J.;Yamada, S.;Murakami, S.
牙周膜（PDL）是维持牙齿和牙周组织内环境稳定的重要因素。铁水平对PDL细胞分化的影响尚未研究，尽管有证据表明铁超载或缺乏会对牙槽骨密度产生不利影响。本研究的目的是研究改变铁水平对人PDL cells.Material and MethodsHuman PDL cells细胞分化的影响，用补充有10-50 μ m柠檬酸铁铵或5 μ m去铁胺（铁螯合剂）的培养基在分化过程中孵育。通过测量铁蛋白RNA和蛋白质表达的变化来评估细胞内铁状态。PDL细胞的分化和功能进行了评估，通过测量成骨细胞分化的基因标记物和能力的文化形成矿化nodules. ResultsFe积累导致上调的轻链和重链铁蛋白。同时，成骨细胞分化基因标记和矿化结节形成受到抑制。铁缺乏导致轻链和重链铁蛋白的下调，抑制碱性磷酸酶的活性和矿化结节的形成过程中PDL细胞differentiation.ConclusionWe得出结论，铁是人类PDL细胞的正常细胞分化的关键。
Background and ObjectiveThe periodontal ligament (PDL) is vital to maintaining the homeostasis of the tooth and periodontal tissue. The influence of iron levels on the cytodifferentiation of PDL cells has not been studied, despite evidence that iron overload or deficiency can have adverse effects on alveolar bone density. The purpose of this study was to examine the effects of altered iron levels on cytodifferentiation in human PDL cells.Material and MethodsHuman PDL cells were incubated with culture media supplemented with 10-50 mu m ammonium ferric citrate or 5 mu m deferoxamine (an iron chelator) during differentiation. Intracellular iron status was assessed by measuring changes in the expression of ferritin RNA and protein. PDL cell differentiation and function were evaluated by measuring osteoblast differentiation gene markers and the capacity of cultures to form mineralized nodules.ResultsIron accumulation resulted in upregulation of light and heavy chain ferritin proteins. Concurrently, osteoblast differentiation gene markers and mineralized nodule formation were suppressed. Iron deficiency resulted in downregulation of light and heavy chain ferritin proteins, suppression of alkaline phosphatase activity and formation of mineralized nodules during PDL cell differentiation.ConclusionWe conclude that iron is critical for normal cell differentiation of human PDL cells.