Resting cytosol Ca2+ level maintained by Ca2+ pumps affects environmental responses in Arabidopsis
Resting cytosol Ca2+ level maintained by Ca2+ pumps affects environmental responses in Arabidopsis
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Ca2泵维持的静息细胞质Ca2水平影响拟南芥的环境反应
DOI：
10.1093/plphys/kiad047
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发表时间：
2023
期刊：
影响因子：
7.4
通讯作者：
Hua, Jian
中科院分区：
文献类型：
--
作者：
Li, Zhan;Harper, Jeffrey F.;Weigand, Chrystle;Hua, Jian
钙离子转运系统控制胞质中Ca 2+水平（[Ca 2 +]cyt），并产生瞬时钙（Ca 2+）信号，这是环境反应的关键。在这里，我们报告的影响，休息[Ca 2 +]胞体植物的钙调素调节的Ca 2+泵或Ca 2 +-ATP酶在拟南芥（拟南芥）的功能研究。质膜定位泵ACA 8（autoinhibited Ca 2 +-ATPase）和ACA 10，以及液泡定位泵ACA 4和ACA 11，在维持低静息[Ca 2 +]cytand植物生存在低温和高温胁迫条件下是至关重要的。它们的功能丧失型mucosaca 8 aca 10和aca 4 aca 11在常温下具有自身免疫性，这种失调的免疫激活被低温增强，导致低温致死。此外，这些突变体还表现出静息[Ca ~（2+）] cyti升高，而外部Ca ~（2+）降低[Ca ~（2+）] cyti，抑制了自身免疫和冷敏感性。茶8 aca 10和茶4 aca 11突变体也对热敏感，可能是由于气孔关闭和叶片表面温度高于野生型。这些观察结果支持了一个模型，在该模型中，静息[Ca 2 +]cytis的调节对植物如何调节生物和非生物反应至关重要。
Calcium ion transporting systems control cytosol Ca2+levels ([Ca2+]cyt) and generate transient calcium (Ca2+) signatures that are key to environmental responses. Here, we report an impact of resting [Ca2+]cyton plants from the functional study of calmodulin-regulated Ca2+pumps or Ca2+-ATPases in Arabidopsis (Arabidopsis thaliana). The plasma membrane-localized pumps ACA8 (autoinhibited Ca2+-ATPase) and ACA10, as well as the vacuole-localized pumps ACA4 and ACA11, were critical in maintaining low resting [Ca2+]cytand essential for plant survival under chilling and heat-stress conditions. Their loss-of-function mutantsaca8 aca10andaca4 aca11had autoimmunity at normal temperatures, and this deregulated immune activation was enhanced by low temperature, leading to chilling lethality. Furthermore, these mutants showed an elevated resting [Ca2+]cyt, and a reduction of external Ca2+lowered [Ca2+]cytand repressed their autoimmunity and cold susceptibility. Theaca8 aca10and theaca4 aca11mutants were also susceptible to heat, likely resulting from more closed stomata and higher leaf surface temperature than the wild type. These observations support a model in which the regulation of resting [Ca2+]cytis critical to how plants regulate biotic and abiotic responses.