COMPOSITION OF INTERSTITIAL CELLULAR INFILTRATE IDENTIFIED BY MONOCLONAL-ANTIBODIES IN RENAL BIOPSIES OF REJECTING HUMAN RENAL-ALLOGRAFTS
COMPOSITION OF INTERSTITIAL CELLULAR INFILTRATE IDENTIFIED BY MONOCLONAL-ANTIBODIES IN RENAL BIOPSIES OF REJECTING HUMAN RENAL-ALLOGRAFTS
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DOI：
10.1097/00007890-198305000-00013
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发表时间：
1983-01-01
期刊：
影响因子：
6.2
通讯作者：
ATKINS, RC
中科院分区：
文献类型：
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作者：
HANCOCK, WW;THOMSON, NM;ATKINS, RC
采用单克隆抗体和4层免疫过氧化物酶技术，对25例急性肾移植排斥反应患者经皮肾穿刺活检中发现的各种浸润细胞类型进行分析和定量。细胞标志物包括抗人白细胞共同抗原（PHM 1）、单核吞噬细胞（PHM 2、FMC 32）、T细胞和T细胞亚群（OKT 3、OKT 4和OKT 8）、B细胞（7.2）、多形细胞（FMC 10、FMC 13）和浆细胞（OKT 10）的单克隆抗体。标记的间质细胞的比例表示为用PHM 1鉴定的浸润性白细胞总数的百分比，并且与排斥的组织学分级强度相关。在轻度排斥反应中，32%的浸润细胞为T淋巴细胞，其中90%为OKT-8阳性细胞毒抑制细胞，52%为巨噬细胞。在中度排斥中，T细胞占浸润的42%（67%的T细胞表达OKT 8抗原），巨噬细胞占总细胞的38%。在严重排斥反应中，T细胞成分减少至15%的细胞，其中78%为OKT-8阳性;这些细胞主要是巨噬细胞（60%）和多形细胞（22%）。显然，细胞毒抑制性T细胞和巨噬细胞是介导急性间质移植排斥反应的主要细胞。
Monoclonal antibodies and a 4-layer immunoperoxidase technique were used to analyze and quantitate the various infiltrating cell types found in percutaneous renal biopsies of 25 patients undergoing acute renal allograft rejection. Cell markers included monoclonal antibodies to the human leukocyte-common antigen (PHM 1), mononuclear phagocytes (PHM 2,FMC 32), T cells and T cell subsets (OKT 3, OKT 4 and OKT 8), B cells (7.2), polymorphs (FMC 10, FMC 13) and plasma cells (OKT 10). The proportion of labeled interstitial cells was expressed as a percentage of the total number of infiltrating leukocytes identified with PHM 1, and correlated with the histologically graded intensity of rejection. In mild rejection 32% of the infiltrating cells were T lymphocytes, of which 90% were OKT-8-positive cytotoxic-suppressor cells, and 52% were macrophages. In moderate rejection T cells composed 42% of the infiltrate (with 67% of T cells expressing OKT 8 antigen), and macrophages formed 38% of the total cells. In severe rejection, the T cell component was decreased to 15% of the cells, of which 78% were OKT-8-positive; these were preponderantly macrophages (60%) and polymorphs (22%). Evidently, cytotoxic-suppressor T cells and macrophages are the major cells mediating acute interstitial graft rejection.