Bacterial adhesion to bisphosphonate coated hydroxyapatite
Bacterial adhesion to bisphosphonate coated hydroxyapatite
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DOI：
10.1007/s10856-005-0625-x
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发表时间：
2005-04-01
影响因子：
3.7
通讯作者：
Grant, MH
中科院分区：
文献类型：
--
作者：
Ganguli, A;Steward, C;Grant, MH
金黄色葡萄球菌（S. aureus）通常与骨科植入物的微生物感染有关。此类感染通常会导致骨髓炎，从而可能因局部骨质破坏而导致植入物失败。手术期间的污染或通过血液循环从远处传播的结果可能会导致装置的细菌粘附和随后的定植。已提出用双膦酸盐氯膦酸盐 (C) 和帕米膦酸盐 (P) 涂覆人工髋关节的羟基磷灰石 (HA) 陶瓷组件，作为尽量减少骨质溶解并从而防止植入物松动的方法。然而，在实施这种方法之前，必须确定双膦酸盐涂层对细菌粘附到 HA 材料的影响。在本研究中，包被的 HA 材料与金黄色葡萄球菌一起孵育，并使用改良涡流装置 (MVD) 方法测定粘附细菌的数量。粘附在 P 涂层 HA 材料上的细菌数量显着大于粘附在未涂层 HA（增加 60 倍）或 C 涂层 HA（增加 90 倍）上的细菌数量。因此，尽管早期研究表明 P 与 HA 结合可能会改善骨整合，但所得出的结果表明，这种涂层的使用可能会受到涂层装置感染敏感性潜在增加的限制。 (C) 2005 年施普林格科学 + 商业媒体公司。
Staphylococcus aureus ( S. aureus) is commonly associated with microbial infection of orthopaedic implants. Such infections often lead to osteomyelitis, which may result in failure of the implant due to localised bone destruction. Bacterial adhesion and subsequent colonisation of the device may occur as a consequence of contamination during surgery, or by seeding from a distant site through the blood circulation. Coating of the hydroxyapatite ( HA) ceramic component of artificial hip joints with the bisphosphonates clodronate ( C) and pamidronate ( P) has been proposed as a means to minimise osteolysis and thereby prevent loosening of the implant. However, the effect of the bisphosphonate coating on bacterial adhesion to the HA materials must be determined before this approach can be implemented. In this study coated HA materials were incubated with the S. aureus and the number of adherent bacteria determined using the Modified Vortex Device (MVD) method. The number of bacteria adherent to the P coated HA material was significantly greater than that adherent to uncoated HA (60-fold increase) or to the C coated HA (90-fold increase). Therefore, even though earlier studies suggested that P bound to HA may improve osseointegration, the results presented would suggest that the use of this coating may be limited by the potential increased susceptibility of the coated device to infection. (C) 2005 Springer Science + Business Media, Inc.