Quantitative and amplification-free detection of SOCS-1 CpG methylation percentage analyses in gastric cancer by fiber optic nanoplasmonic biosensor
Quantitative and amplification-free detection of SOCS-1 CpG methylation percentage analyses in gastric cancer by fiber optic nanoplasmonic biosensor
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DOI：
10.1016/j.bios.2022.114540
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发表时间：
2022-07-11
影响因子：
12.6
通讯作者：
Lin，Shu-Hui
中科院分区：
文献类型：
--
作者：
Guthula，Lakshmi Sujatha;Yeh，Kun-Tu;Lin，Shu-Hui
一种新的创新方法对于胃癌的早期有效诊断至关重要，该方法利用肿瘤抑制因子SOCS-1的启动子超甲基化，SOCS-1在人类癌症中经常失活。我们开发了一种无扩增光纤纳米等离子体生物传感器，用于检测socs -1人类基因组的DNA甲基化。该方法是基于从人胃肿瘤组织和细胞系中提取无pcr DNA的光纤纳米金连接吸附剂测定。我们设计了一个特定的DNA探针，制作在纤维芯表面，而另一个探针与自由形式的金纳米颗粒生物偶联，以确定百分比并区分甲基化和未甲基化的细胞系，进一步证明了ocs -1甲基化发生在癌症患者中，而不是在正常细胞系中。甲基化DNA的检出限为0.81 fM，检测时间在15 min以内。此外，我们的数据与基于pcr的焦磷酸测序获得的数据显著相关，但具有更高的准确性。因此，我们的研究结果为人类基因组甲基化状态的定量评估提供了新的见解，并且可以作为PCR的替代品，具有很大的潜力。
A new innovative approach is essential for early and effective diagnosis of gastric cancer, using promoter hypermethylation of the tumor suppressor,SOCS-1, that is frequently inactivated in human cancers. We have developed an amplification-free fiber optic nanoplasmonic biosensor for detecting DNA methylation of theSOCS-1human genome. The method is based on the fiber optic nanogold-linked sorbent assay of PCR-free DNA from human gastric tumor tissue and cell lines. We designed a specific DNA probe fabricated on the fiber core surface while the other probe is bioconjugated with gold nanoparticles in free form to allow percentage determination and differentiating the methylated and unmethylated cell lines, further demonstrating theSOCS-1methylation occurs in cancer patients but not in normal cell lines. The observed detection limit is 0.81 fM for methylated DNA, and the detection time is within 15 min. In addition, our data were significantly correlated to the data obtained from PCR-based pyrosequencing, and yet with superior accuracy. Hence our results provide new insight to the quantitative evaluation of methylation status of the human genome and can act as an alternative to PCR with a great potential.