Deep sequencing reveals specific bacterial signatures in the subgingival microbiota of healthy subjects
Deep sequencing reveals specific bacterial signatures in the subgingival microbiota of healthy subjects
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DOI：
10.1007/s00784-019-02805-3
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发表时间：
2019-03-01
影响因子：
3.4
通讯作者：
Izumi, Yuichi
中科院分区：
文献类型：
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作者：
Ikeda, Eri;Shiba, Takahiko;Izumi, Yuichi
目的本研究旨在确定全面的细菌植物群的健康口腔通过鉴定和比较不同的龈下部位的细菌种类，使用454 16S rRNA基因测序的材料和方法龈下菌斑样品取自6个目标牙齿（中切牙，第一前磨牙，第一磨牙在上，下颌骨）的10名牙周健康患者。细菌DNA提取和16S rRNA基因的下一代测序performed.ResultsBacterial组合物在门的水平是相似的所有网站在同一个人的牙齿位置无关。未加权的UniFrac距离值的微生物组也表明，平均距离显着大于受试者之间的牙齿位置的相同subjects.ConclusionsThe目前的结果澄清缺乏牙齿位置的影响，在健康的龈下菌群。结果可能表明，任何龈下部位都可以表现出类似的受试者特异性microbiota.Clinical relevanceThis调查提供了一个更好的了解口腔微生物组的独特性。本研究将有助于未来的龈下微生物研究的采样。
ObjectivesThis study aimed to define the comprehensive bacterial flora of the healthy oral cavity by identifying and comparing bacterial species in different subgingival sites using 454 sequencing of 16S rRNA genes.Materials and methodsSubgingival plaque samples were taken from six target teeth (central incisor, first premolar, and first molar in both the maxilla and mandible) of 10 periodontally healthy patients. Bacterial DNA was extracted and next-generation sequencing of 16S rRNA genes was performed.ResultsBacterial composition in phylum level was similar for all sites within the same individual irrespective of tooth location. Unweighted UniFrac distance values of microbiome also showed that average distance was significantly larger between subjects than between tooth locations of the same subjects.ConclusionsThe present results clarify the lack of effect of tooth location in the healthy subgingival microbiota. Results may suggest that any subgingival site can demonstrate similar subject-specific microbiota.Clinical relevanceThis investigation offers a better understanding of the uniqueness of the oral microbiome. The present study will facilitate sampling in future subgingival microbiological studies.