Ethanol administration impairs pancreatic repair after injury.
Ethanol administration impairs pancreatic repair after injury.
复制标题
DOI：
10.1097/mpa.0b013e31824bde37
复制
发表时间：
2012-11
期刊：
影响因子：
2.9
通讯作者：
Clemens DL
中科院分区：
文献类型：
--
作者：
Schneider KJ;Scheer M;Suhr M;Clemens DL
酒精滥用是与急性和慢性胰腺炎相关的最常见因素之一。虽然酒精滥用在胰腺炎的发展中起着重要作用，但似乎酒精滥用本身并不是这种疾病的原因。我们研究了酒精在胰腺炎诱导后损伤胰腺修复中的作用。本研究采用慢性酒精摄入和柯萨奇病毒感染相结合的酒精性胰腺炎小鼠模型，研究酒精对胰腺再生的影响。收获组织并通过RT-PCR和免疫印迹分析。这些研究表明，慢性乙醇消耗损害外分泌胰腺的结构修复。这伴随着脂肪酶表达恢复的延迟。此外，观察到关键胰腺转录因子PDX 1和PTF 1以及Notch信号传导介质HES 1的表达受损。慢性乙醇消耗损害严重损伤后胰腺的结构修复和功能恢复。这些损伤可能部分地由外分泌胰腺的发育和维持中重要的因子的表达受损来解释。胰腺再生受损可能在酒精性胰腺炎的发病机制中起作用。
Alcohol abuse is one of the most common factors associated with acute and chronic pancreatitis. Although it is evident that alcohol abuse can have an important role in the development of pancreatitis, it does not appear that alcohol abuse alone is responsible for this disease. We investigated the involvement of ethanol in impairment of pancreatic repair after induction of pancreatitis. A biologically relevant mouse model of alcoholic pancreatitis, combining chronic ethanol consumption and coxsackievirus infection, was used to investigate the effects of ethanol on pancreatic regeneration. Tissues were harvested and analyzed by RT-PCR and immunoblot. These studies demonstrate that chronic ethanol consumption impairs the structural repair of the exocrine pancreas. This is accompanied by a delay in the restitution of lipase expression. Additionally, impaired expression of the critical pancreatic transcription factors, PDX1 and PTF1, and the mediator of Notch signaling, HES1, were observed. Chronic ethanol consumption impairs the structural repair and functional restitution of the pancreas after severe injury. These impairments may, in part, be explained by impaired expression of factors important in the development and maintenance of the exocrine pancreas. Impaired pancreatic regeneration may have a role in the pathogenesis of alcoholic pancreatitis.