Effects of photodynamic therapy on Streptococcus mutans and enamel remineralization of multifunctional TiO2-HAP composite nanomaterials
Effects of photodynamic therapy on Streptococcus mutans and enamel remineralization of multifunctional TiO2-HAP composite nanomaterials
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DOI：
10.1016/j.pdpdt.2022.103141
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发表时间：
2023-04-05
影响因子：
3.3
通讯作者：
Pan, Shuang
中科院分区：
文献类型：
--
作者：
Wang, Ranxu;Jia, Conghui;Pan, Shuang
背景：二氧化钛（TiO 2）作为光敏剂和光催化剂，能产生光动力学反应，用于抗菌处理。本研究旨在探索一种二氧化钛/纳米羟基磷灰石（TiO 2-HAP）复合材料与牙科固化灯（385-515 nm）联合应用于临床，抑制变形链球菌（S. mutans），同时促进釉质表面的矿化。研究方法：采用X射线衍射（XRD）和高分辨透射电子显微镜（HRTEM）对TiO 2-HAP复合纳米材料进行了表征。利用漫反射光谱和荧光光谱研究了TiO 2-HAP的光动力学性质。通过阅读细菌培养物的吸光度来测量细菌生长，并使用共聚焦显微镜观察纳米材料的生物膜去除能力。扫描电镜观察TiO 2-HAP促进釉质矿化的能力。在150 mW/cm 2的LED照射下，TiO 2-HAP组的抗菌活性为0.13，表明其具有持续的抗菌作用和对菌斑微生物膜代谢活性的抑制作用。TiO_2-HAP中钙、磷离子的释放可同时促进釉质矿化。结论：TiO 2-HAP具有良好的抗菌和矿化能力，能最大限度地预防龋病的发生，促进牙组织仿生矿化。
Background: As photosensitizer and photocatalyst, titanium dioxide (TiO2) can produce a photodynamic reaction for antibacterial treatment. This study aims to explore a Titanium dioxide/nano-hydroxyapatite (TiO2-HAP) composite combined with the dental curing lamp (385-515 nm) in clinical which could inhibit the dental plaque biofilm formed by Streptococcus mutans (S. mutans) and promote the enamel surface remineralization simultaneously. Methods: X-ray Diffraction (XRD) and high resolution transmission electron microscope (HRTEM) were used to detect the characterization of TiO2-HAP composite nanomaterials. Photodynamic properties of TiO2-HAP were detected by Diffuse reflectance spectrum (DRS) and fluorescence spectroscopy. Bacterial growth was measured by reading the absorbance of bacterial cultures and confocal microscope was used to observe the biofilm removal ability of nanomaterials. The ability of TiO2-HAP to promote enamel remineralization was measured by Scanning electron microscope (SEM).Results: The OD 600 of S. mutans was 0.76 in the control group and 0.13 in group of TiO2-HAP with exposure to light-emitting diode (LED) (150 mW/cm2) for 5 min, suggesting its sustained antibacterial potency and inhibition of the metabolic activity of dental plaque microcosm biofilm. Also, the release of calcium and phosphorus ions in TiO2-HAP can promote enamel mineralization simultaneously. After 15 days of remineralization, the Ca/P ratio of demineralized enamel surface increased from 1.28 to 1.67, which was similar to that of normal enamel.Conclusions: The TiO2-HAP exhibit a promising anti-bacterial activity and remineralization capacity which can prevent the occurrence of caries to the greatest extent and promote the biomimetic mineralization of dental tissues.