Enhanced resistance to fungal pathogens in transgenic Populus tomentosa Carr. by overexpression of an nsLTP-like antimicrobial protein gene from motherwort (Leonurus japonicus)
Enhanced resistance to fungal pathogens in transgenic Populus tomentosa Carr. by overexpression of an nsLTP-like antimicrobial protein gene from motherwort (Leonurus japonicus)
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转基因毛白杨 Carr 对真菌病原体的抵抗力增强。
DOI：
10.1093/treephys/tpq093
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发表时间：
2010-12-01
期刊：
影响因子：
4
通讯作者：
Luo, Keming
中科院分区：
文献类型：
--
作者：
Jia, Zhichun;Gou, Jiqing;Luo, Keming
抗菌蛋白基因LJAMP2是益母草（Leonurus）的一种植物非特异性脂质转运蛋白。本研究以毛白色白杨（Populus tomentosa卡尔.）通过农杆菌介导的转化方法，将卡那霉素抗性基因转化到农杆菌中。共获得16个白杨品系，聚合酶链反应（PCR）分析建立了稳定的植物基因组中的转基因整合。反转录PCR检测到LJAMP2在转基因植株中的表达。对烟草赤星病菌（Alternaria alternata（Fr.）Keissler和胶孢炭疽菌Colletotrichum gloeosporioides（Penz.）对转基因白杨品系进行了检测。从转化体的粗叶提取物中可以明显看出对真菌病原体的体外抑制活性。体内试验表明，感染A. alternata（Fr.）Keissler和C. gloeosporioides（Penz.），与对照相比，转基因白杨植物中的疾病症状显著减轻。这些结果表明，益母草LJAMP2基因的组成型表达可以用来提高白杨对真菌病原菌的抗性。
The antimicrobial protein gene LJAMP2 is a plant non-specific lipid transfer protein from motherwort (Leonurus japonicus). In this study, it was introduced into Chinese white poplar (Populus tomentosa Carr.) via Agrobacterium-mediated transformation with neomycin phosphotransferase II gene conferring kanamycin resistance as selectable marker. A total of 16 poplar lines were obtained, and polymerase chain reaction (PCR) analysis established the stable integration of transgenes in the plant genome. Reverse transcription-PCR detected LJAMP2 expression in transgenic plants. Resistance to fungal pathogens Alternaria alternata (Fr.) Keissler and Colletotrichum gloeosporioides (Penz.) of transgenic poplar lines was tested. In vitro inhibitory activity against the fungal pathogens was evident from the crude leaf extracts from the transformants. In vivo assays showed that, after infection with both A. alternata (Fr.) Keissler and C. gloeosporioides (Penz.), there was a significant reduction in disease symptoms in transgenic poplar plants compared with the control. These results suggest that constitutive expression of the LJAMP2 gene from motherwort can be exploited to improve resistance to fungal pathogens in poplar.