Effect of Shoot Age and Tip Culture Propagation of Grapes on Systemic Infestations byAgrobacterium tumefaciensBiovar 3
Effect of Shoot Age and Tip Culture Propagation of Grapes on Systemic Infestations byAgrobacterium tumefaciensBiovar 3
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葡萄芽龄和芽尖培养繁殖对根癌农杆菌 Biovar 3 系统侵染的影响
DOI：
10.5344/ajev.1988.39.1.67
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发表时间：
1988
影响因子：
1.9
通讯作者：
V. Mittak
中科院分区：
文献类型：
--
作者：
T. Burr;B. H. Katz;A. L. Bishop;C. Meyers;V. Mittak
根癌农杆菌生物变种 3 (AT3) 是导致葡萄冠瘿的原因，在 8 月下旬和 10 月收集的葡萄芽中系统检测到，但在 5 月、7 月或 8 月初收集的绿芽中未检测到。在温室实验中，病原体并未在一个月内从甘蔗上的接种点短距离迁移到绿芽中。通过茎尖培养产生的藤蔓不含 AT3，无论最初的茎尖是取自已知感染 AT3 的植物还是表面上干净的植物。通过茎尖培养繁殖的藤蔓在温室中的组织培养基或盆栽混合物中生长六个月后仍不含病原体。
Agrobacterium tumefaciens biovar 3 (AT3), the cause of grape crown gall, was detected systemically in grape shoots that were collected in late August and October but not in green shoots collected in May, July, or early August. The pathogen did not migrate short distances from inoculation points on canes into green shoots within one month in greenhouse experiments. Vines produced by shoot tip culture were free of AT3 regardless of whether the original tips were taken from shoots of known AT3-infested or apparently clean plants. Vines propagated by shoot tip culture remained free of the pathogen when grown on tissue culture media or in a potting mixture in the greenhouse for six months.