Visualization of pulmonary clearance mechanisms via noninvasive optical imaging validated by near-infrared flow cytometry.
Visualization of pulmonary clearance mechanisms via noninvasive optical imaging validated by near-infrared flow cytometry.
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通过近红外流式细胞术验证的无创光学成像可视化肺部清除机制。
DOI：
10.1002/cyto.a.22658
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发表时间：
2015
期刊：
影响因子：
--
通讯作者：
Berezin,MikhailY
中科院分区：
文献类型：
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作者：
Zhou,Haiying;Gunsten,SeanP;Zhegalova,NataliaG;Bloch,Sharon;Achilefu,Samuel;ChristopherHolley,J;Schweppe,Daniel;Akers,Walter;Brody,StevenL;Eades,WilliamC;Berezin,MikhailY
使用近红外（NIR）探针的体内光学成像是临床前和临床研究中已确立的诊断方法。然而，由于缺乏NIR流式细胞术，这些探针的特异性难以离体验证。为了解决这一限制，我们修改了流式细胞仪，以包括使用752 nm激光线的额外NIR通道。使用可见光范围和NIR荧光染料的组合标记的NIR微球和细胞系测试流式细胞术系统。该方法在小鼠体内进行了验证，评价了NIR造影剂的肺内递送后肺部的免疫应答。从肺支气管肺泡灌洗获得的细胞的流式细胞术证明，NIR染料早在注射后4 h就被肺巨噬细胞摄取。光学成像与NIR流式细胞术的这种组合扩展了成像能力，并使体内成像与细胞特异性研究互补。© 2015国际细胞计数促进学会
In vivo optical imaging with near‐infrared (NIR) probes is an established method of diagnostics in preclinical and clinical studies. However, the specificities of these probes are difficult to validate ex vivo due to the lack of NIR flow cytometry. To address this limitation, we modified a flow cytometer to include an additional NIR channel using a 752 nm laser line. The flow cytometry system was tested using NIR microspheres and cell lines labeled with a combination of visible range and NIR fluorescent dyes. The approach was verified in vivo in mice evaluated for immune response in lungs after intratracheal delivery of the NIR contrast agent. Flow cytometry of cells obtained from the lung bronchoalveolar lavage demonstrated that the NIR dye was taken up by pulmonary macrophages as early as 4‐h post‐injection. This combination of optical imaging with NIR flow cytometry extends the capability of imaging and enables complementation of in vivo imaging with cell‐specific studies. © 2015 International Society for Advancement of Cytometry