Cryptotanshinone attenuates allergic airway inflammation through negative regulation of NF-κB and p38 MAPK
Cryptotanshinone attenuates allergic airway inflammation through negative regulation of NF-κB and p38 MAPK
复制标题
隐丹参酮通过 NF-kappa B 和 p38 MAPK 的负调节减轻过敏性气道炎症
DOI：
10.1080/09168451.2019.1687280
复制
发表时间：
2019-11-08
影响因子：
1.6
通讯作者：
Jin, Yongde
中科院分区：
文献类型：
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作者：
Li, Junfeng;Zheng, Mingyu;Jin, Yongde
本研究旨在探讨隐丹参酮(CTS)在过敏性呼吸道炎症中的作用及机制。采用BALB/c小鼠建立卵清蛋白致敏哮喘模型。肺组织中IL-6、IL-13、IL-4、IL-5、IL-6和IL-13水平升高，肺组织中总免疫球蛋白E(IgE)、OVA特异性IgE、嗜酸性粒细胞趋化因子、ICAM-1、VCAM-1、核因子-kappaB(NF-kappaB)和p38MAPK磷酸化水平升高。然而，CTS显著降低哮喘小鼠的AHR，减轻细支气管炎性反应和气道周围炎症细胞，调节细胞因子的产生，降低总IgE和OVA特异性IgE水平，并抑制NF-kappaB活化和p38MAPK磷酸化。在16个HBE细胞的体外实验表明，CTS降低了CAM-1和IL-6的表达。这些结果表明，CTS通过调节p38MAPK的磷酸化和核因子-kappaB的激活来减轻过敏性气道炎症。
This study is to determine the role and mechanism of cryptotanshinone (CTS) in allergic airway inflammation. Asthma induced by OVA was established in BALB/c mice. We found increased airway hyperresponsiveness (AHR), increased inflammatory cell infiltration, elevated levels of TNF-alpha, interleukin-1 beta (IL-1 beta), IL-4, IL-5, IL-6 and IL-13, decreased interferon gamma (IFN-gamma) in lung tissue, increased content of total immunoglobulin E (IgE), OVA specific IgE, Eotaxin, ICAM-1, VCAM-1, nuclear factor-kappaB (NF-kappa B) and phosphorylation of p38 MAPK in lung tissue. However, the administration of CTS significantly decreased AHR in asthmatic mice, reduced inflammation around the bronchioles and inflammatory cells around airway, regulated cytokine production, reduced the total IgE and OVA-specific IgE levels, and inhibited NF-kappa B activation and p38 MAPK phosphorylation. In vitro experiments in 16 HBE cells revealed that CTS attenuated CAM-1 and IL-6 expression. These results indicate that CTS alleviates allergic airway inflammation by modulating p38 MAPK phosphorylation and NF-kappa B activation.