Genetic variability and forensic efficiency of 39 microsatellite loci in the Li ethnic group from Hainan Island in the South China Sea
Genetic variability and forensic efficiency of 39 microsatellite loci in the Li ethnic group from Hainan Island in the South China Sea
复制标题
南海海南岛黎族39个微卫星位点的遗传变异及法医效率.
DOI：
10.1080/03014460.2016.1241300
复制
发表时间：
2017-01-01
影响因子：
1.7
通讯作者：
Yan, Jiangwei
中科院分区：
文献类型：
--
作者：
Chen, Jing;Xie, Bingbing;Yan, Jiangwei
背景资料：研究黎族人群39个常染色体短串联重复序列（STR）基因座的群体遗传学数据和法医学参数，探讨黎族人群与其他参考群体的遗传关系。D19S433、D5S818、D21S11、D18S51、D6S1043、D3S1358、D13S317、D7S820、D16S539、CSF 1PO、Penta D、D2S441、vWA、D8S1179、TPOX、Penta E、TH 01、D12S391、D2S1338、FGA、D 6S 477、D18 S535、D19 S253、D15 S659、D11 S2368、D20 S470、D1 S1656、D22-GATA 198 B 05、D8 S1132、D4 S2366、D21 S1270、D13 S325、D9 S925、D3 S3045、D14 S608、D10 S1435、D 7S 3048、D17 S1290和D5 S2500，用两种多重DNA-STR荧光检测系统对189名黎族健康个体进行扩增。结果：共观察到378个等位基因，对应的等位基因频率范围为0.0026 ~ 0.5899。辨别力和排除力分别为0.7569-0.9672和0.2513-0.7355。排除率（PE）三亲关系为0.2580-0.7943，二亲关系为0.1693-0.5940。多态信息含量（PIC）在0.5001 ~ 0.8611之间。39个STR基因座的累积匹配概率为2.4242 × 10（-38）。结论：海南黎族人群中39个STR基因座具有多态性。这组多态性STR基因座为法医学个体识别和亲子鉴定提供了高度多态性的信息和法医学效率，也为群体遗传学和人类学研究提供了基础群体数据。
Background: Investigation of allele and genotype frequencies of microsatellite loci in various populations is an essential pre-requisite in forensic application.Aim: The present study obtained population genetic data and forensic parameters of 39 autosomal Short Tandem Repeats (STRs) loci from a Chinese Li ethnic group and estimated the genetic relationships between Li and other reference populations.Subjects and methods: Thirty-nine STR loci, which include D19S433, D5S818, D21S11, D18S51, D6S1043, D3S1358, D13S317, D7S820, D16S539, CSF1PO, Penta D, D2S441, vWA, D8S1179, TPOX, Penta E, TH01, D12S391, D2S1338, FGA, D6S477, D18S535, D19S253, D15S659, D11S2368, D20S470, D1S1656, D22-GATA198B05, D8S1132, D4S2366, D21S1270, D13S325, D9S925, D3S3045, D14S608, D10S1435, D7S3048, D17S1290 and D5S2500, were amplified in two multiplex DNA-STR fluorescence detection systems for 189 unrelated healthy individuals of the Chinese Li ethnic group. The allele frequency distribution and several parameters commonly used in forensic science were statistically analysed.Results: A total of 378 alleles were observed with corresponding allelic frequencies ranging from 0.0026-0.5899. The power of discrimination and power of exclusion ranged from 0.7569-0.9672 and 0.2513-0.7355, respectively. The power of exclusion (PE) ranged from 0.2580-0.7943 for trio paternity cases and 0.1693-0.5940 for duo paternity cases. The polymorphism information content (PIC) ranged from 0.5001-0.8611. The cumulative match probability across these 39 loci was 2.4242x10(-38).Conclusion: The results indicate that 39 STR loci are polymorphic among the Li ethnic group in Hainan Island in the South China Sea. This set of polymorphic STR loci provide highly polymorphic information and forensic efficiency for forensic individual identification and paternity testing, as well as basic population data for population genetics and anthropological research.