Purification and Characterization of Feruloyl Esterase from Aspergillus awamori
Purification and Characterization of Feruloyl Esterase from Aspergillus awamori
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泡盛曲霉阿魏酸酯酶的纯化和表征
DOI：
10.3136/fstr.5.251
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发表时间：
1999
影响因子：
0.6
通讯作者：
S. Toyama
中科院分区：
文献类型：
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作者：
M. Ishihara;Naoko Nakazato;Kunio Chibana;S. Tawata;S. Toyama
采用硫酸铵分馏法、DEAE-Sephadex A-50柱层析、DEAE-Toyopearl 650柱层析、Butyl Toyopearl 650S疏水层析和Toyopearl HW55凝胶过滤纯化A. awamori阿铁酸酯酶FAE-a和FAE-b。SDS-PAGE估计FAE-a和FAE-b的分子量（Mr）分别为31 KDa和29 KDa；这些等电点分别为3.9和3.4以下。两种酶在45 ~ 50℃、pH 5.0 ~ 5.5条件下活性最佳。FAE-a在37℃下稳定，FAE-b在50℃下稳定。这两种酶主要水解由菠萝茎渣制备的阿魏酸化碳水化合物酯，而不是阿魏酸甲酯。
A. awamori feruloly esterases, termed FAE-a and FAE-b, were purified to homogeneity by ammonium sulfate fractionation, DEAE-Sephadex A-50 column chromatography, DEAE-Toyopearl 650 column chromatography, hydrophobic chromatography on Butyl Toyopearl 650S, and gel filtration on Toyopearl HW55. The molecular weights (Mr) of FAE-a and FAE-b were estimated to be 31 KDa and 29 KDa by SDS-PAGE; these isoelectric points were 3.9 and below 3.4, respectively. Both enzymes were optimally active at 45-50°C and pH 5.0-5.5. FAE-a was stable up to 37°C, and FAE-b was stable up to 50°C. Both enzymes hydrolyzed predominantly the feruloylated carbohydrate ester prepared from pineapple stem residue rather than methyl esters of ferulic acid.
A.Abe：“猪霍乱沙门氏菌血清型霍乱沙门氏菌毒力质粒 pKDS50 的 spvR 基因表达的调节”分子微生物学。
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Koseki：“衣藻营养细胞周期 I 中子细胞释放的控制。孵化酶的纯化及其作为 119 kDa 蛋白酶的表征”
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