Expression characteristics of the SUMOylation genes SUMO-1 and Ubc9 in the developing testis and ovary of Chinese mitten crab, Eriocheir sinensis.
Expression characteristics of the SUMOylation genes SUMO-1 and Ubc9 in the developing testis and ovary of Chinese mitten crab, Eriocheir sinensis.
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DOI：
10.1016/j.gene.2012.04.016
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发表时间：
2012-06
期刊：
影响因子：
3.5
通讯作者：
Qun Wang;Ying Wang;Lili Chen;Lin He;Weiwei Li;Hui Jiang
中科院分区：
文献类型：
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作者：
Qun Wang;Ying Wang;Lili Chen;Lin He;Weiwei Li;Hui Jiang
真核生物中的小泛素样修饰剂 (SUMO) 途径是多种细胞过程、发育和细胞器生物发生所需的重要翻译后修饰。 SUMO结合酶（Ubc9）是SUMO途径中重要的结合酶。 SUMO-1 和 Ubc9 已在脊椎动物中发现；然而，它们在甲壳类动物中的表达却知之甚少。本研究在中华绒螯蟹发育中的睾丸和卵巢中鉴定出了SUMO-1和Ubc9基因，并分别命名为EsSUMO-1和EsUbc9。实时定量 PCR 表明，两种 mRNA 的表达水平在睾丸和卵巢发育过程中存在显着差异。在睾丸中，EsSUMO-1和EsUbc9在II-1期中等水平表达，在II-2期增加，然后在IV期逐渐下降。卵巢中EsSUMO-1和EsUbc9的表达量在早期较低,在III-2期达到最高水平,然后在IV期逐渐下降。使用原位杂交在整个睾丸和卵巢、精子细胞和卵母细胞中检测到这两个基因的转录本。 EsSUMO-1 和 EsUbc9 在睾丸和卵巢中的表达模式表明 SUMO 化可能在中华鲟的精子发生和卵子发生中发挥重要作用。
The small ubiquitin-like modifier (SUMO) pathway in eukaryotes is an essential post-translational modification required for a variety of cellular processes, development and organelle biogenesis. SUMO-conjugating enzyme (Ubc9) is an important conjunction enzyme in the SUMO pathway. SUMO-1 and Ubc9 have been found in vertebrates; however, their expression in crustaceans was poorly characterized. In this study, the SUMO-1 and Ubc9 genes were identified in the developing testis and ovary of Chinese mitten crab, Eriocheir sinensis, and designated EsSUMO-1 and EsUbc9, respectively. Quantitative real-time PCR demonstrated the expression level of both mRNAs varied significantly during testis and ovary development. In the testis, EsSUMO-1 and EsUbc9 were expressed at moderate levels in stage II-1, increased at stage II-2, and then gradually declined in stage IV. In the ovary, EsSUMO-1 and EsUbc9 expression were low in the early stage, reach the highest level at stage III-2, and then gradually decreased in stage IV. Transcripts from both genes were detected using in situ hybridization throughout the testis and ovary, in the spermatids and oocytes. The pattern of EsSUMO-1 and EsUbc9 expression in the testis and ovary suggests that SUMOylation may play an important role in spermatogenesis and oogenesis in E. sinensis.