Yeast mitochondrial division and distribution require the cortical Num1 protein
Yeast mitochondrial division and distribution require the cortical Num1 protein
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DOI：
10.1016/j.devcel.2007.01.017
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发表时间：
2007-03-01
影响因子：
11.8
通讯作者：
Sesaki, Hiromi
中科院分区：
文献类型：
--
作者：
Cerveny, Kara L.;Studer, Seth L.;Sesaki, Hiromi
酵母线粒体的分裂需要动力蛋白相关的DNM1蛋白。通过分离显性负Dnm1p突变体的高拷贝抑制子，我们揭示了Num1p在线粒体分裂和遗传中意想不到的作用，Num1p是一种先前被证明促进核迁移的蛋白质。Num1突变体包含一个相互连接的线粒体管网络，与缺乏Dnm1p的细胞非常相似，时间推移显微镜证实Num1 Delta细胞的线粒体分裂大大减少。我们还发现Num1p组装成点状结构，经常与线粒体结合的Dnm1p颗粒共存。我们发现Num1DNM1双突变体积累线粒体，提示Num1p和Dnm1p在线粒体遗传中都起作用。在子芽中，母细胞通常缺乏所有线粒体。因此，我们的研究揭示了Dnm1p通过与Num1p相互作用在线粒体传递中的额外作用，从而在线粒体分裂和遗传之间提供了联系。
Yeast mitochondrial division requires the dynamin-related Dnm1 protein. By isolating high-copy suppressors of a dominant-negative Dnm1p mutant, we uncovered an unexpected role in mitochondrial division and inheritance for Num1p, a protein previously shown to facilitate nuclear migration. num1 mutants contain an interconnected network of mitochondrial tubules, remarkably similar to cells lacking Dnm1p, and time-lapse microscopy confirms that mitochondrial fission is greatly reduced in num1 Delta cells. We also find that Num1p assembles into punctate structures, which often colocalize with mitochondrial-bound Dnm1p particles. Suggesting a role for both Num1p and Dnm1p in mitochondrial inheritance, we find that num1 dnm1 double mutants accumulate mitochondria. in daughter buds and that mother cells are frequently devoid of all mitochondria. Thus, our studies have revealed an additional role for Dnm1p in mitochondrial transmission through its interaction with Num1p, thereby providing a link between mitochondrial division and inheritance.