Fetal Environment and Glycosylation Status in Neonatal Cord Blood: A Comprehensive Mass Spectrometry-based Glycosylation Analysis.
Fetal Environment and Glycosylation Status in Neonatal Cord Blood: A Comprehensive Mass Spectrometry-based Glycosylation Analysis.
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DOI：
10.1097/md.0000000000003219
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发表时间：
2016-04
期刊：
影响因子：
1.6
通讯作者：
HBC study group
中科院分区：
文献类型：
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作者：
Sato R;Tsuchiya KJ;Matsuzaki H;Takei N;Itoh H;Kanayama N;Suda T;Watanabe H;Ohashi T;Tanaka M;Nishimura SI;Maekawa M;HBC study group
补充数字内容可在正文中找到胎儿环境是2型糖尿病和心血管疾病的主要预测因素。然而，胎儿环境和脐带血糖型之间的关系尚不确定。在这项研究中，我们的目的是确定新生儿脐带血中的糖基化状态是否与反映不良胎儿环境的围产期结局有关。从纵向出生队列中招募了36名低出生体重(LBW)婴儿和120名正常出生体重婴儿。我们使用基质辅助激光解吸/电离飞行时间质谱仪进行了全面的脐带血N-葡聚糖分析。用Logistic回归或多元回归分析N-糖链与围产儿结局的关系，包括LBW、小于胎龄以及脐血瘦素和脂联素水平。我们还前瞻性地探讨了N-糖链与6个月或18个月快速体重增加(>；0.67 SD评分)之间的相关性。共检测到35个N-糖链(m/z值1362.481~3865.407)。其中，G3414的丰度水平(m/z值3414.238)与LBW呈负相关，与胎龄的相关性较小。G1915(m/z值1914.698)、G2744(m/z值2743.994)、G3049(m/z值3049.105)和G3719(m/z值3719.349)的丰度水平与低体重负相关。脐血总N-糖链丰度水平与瘦素、脂联素水平呈显著正相关。在一项前瞻性的探索性分析中，5种与LBW相关的N-糖链(G1915、G2744、G3049、G3414和G3719)都与6个月或18个月的快速体重增加呈负相关。这些N-糖链在结构上分为两类：岩藻糖化双或三触角N-糖链；以及无岩藻糖基化的三触角或四触角N-糖链。总而言之，基于质谱学的脐带血糖基化分析表明，5种类型的N-糖链是预测不良胎儿环境的潜在指标。
Supplemental Digital Content is available in the text Fetal environment is known to be a major predictive factor of type 2 diabetes and cardiovascular disease. However, associations of fetal environment and cord blood glycoforms are uncertain. In this study, we aimed to determine whether glycosylation status in neonatal cord blood is associated with perinatal outcomes reflecting a poor fetal environment. Thirty-six low birth weight (LBW) infants and 120 normal birth weight infants were recruited from a longitudinal birth cohort. We conducted a comprehensive cord blood N-glycan analysis using matrix-assisted laser-desorption/ionization time-of-flight mass spectrometry. Associations of N-glycans with perinatal outcomes, including LBW, small for gestational age, and levels of cord blood leptin and adiponectin, were evaluated using logistic or multiple regression. We also prospectively explored correlations between N-glycans and 6 or 18-month rapid weight gain (>0.67 SD score). A total of 35 N-glycans were detected (m/z value 1362.481–3865.407). Of these, abundance levels of G3414 (m/z value 3414.238) were inversely correlated with LBW and small for gestational age. Abundance levels of G1915 (m/z value 1914.698), G2744 (m/z value 2743.994), G3049 (m/z value 3049.105), and G3719 (m/z value 3719.349) were inversely related to LBW. The total N-glycan abundance levels were strongly positively correlated with levels of leptin and adiponectin in cord blood. In a prospective exploratory analysis, the 5 LBW-related N-glycans (G1915, G2744, G3049, G3414, and G3719) were all inversely associated with 6 or 18-month rapid weight gain. These N-glycans are structurally categorized into 2 different categories: fucosylated bi or tri-antennary N-glycans; and tri or tetra-antennary N-glycans without fucosylation. In conclusion, mass spectrometry-based cord blood glycosylation analysis shows that 5 types of N-glycans are potential predictors of a poor fetal environment.