Human milk-derived oligosaccharides and plant-derived oligosaccharides stimulate cytokine production of cord blood T-cells in vitro
Human milk-derived oligosaccharides and plant-derived oligosaccharides stimulate cytokine production of cord blood T-cells in vitro
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DOI：
10.1203/01.pdr.0000139411.35619.b4
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发表时间：
2004-10-01
影响因子：
3.6
通讯作者：
Szépfalusi, Z
中科院分区：
文献类型：
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作者：
Eiwegger, T;Stahl, B;Szépfalusi, Z
母乳中含有大量游离低聚糖(HMOS)。HMOS已被证明具有抗炎作用，其免疫调节作用的证据也越来越多。本研究的目的是评价两种人乳寡糖样品(中性和酸性低聚糖)和一种低分子岩藻糖胶对脐血单个核细胞细胞因子产生和活化的影响。随机选取健康新生儿脐血单个核细胞与寡糖样品共培养。用流式细胞仪检测细胞内细胞因子的产生(D20)和T细胞表面标志的表达(D5)。用散射比浊法(总IgG1)和酶联免疫吸附试验(总IgE)测定体外诱导的细胞培养上清液中Ig水平。酸性低聚糖组分可增加CD3+CD4+和CD3+CD8+细胞产生干扰素-γ的百分率(p<；0.05)和CD3+CD8+细胞产生IL-13的百分率(p<；0.05)。在酸性寡糖培养条件下，CD3+CD4+细胞表面CD25+的表达显著升高(p<；0.05)。低分子岩藻糖胶可诱导CD3+CD4+T细胞产生IL-4(p<；0.05)和CD3+CD8+T细胞产生IL-13(p<；0.05)，而干扰素-γ的产生在这两种T细胞群中仍未受到影响。免疫球蛋白产生(总IgE和总IgG1)未受影响。人乳源低聚糖和植物源低聚糖在体外影响脐带血T细胞细胞因子的产生和激活。因此，低聚糖，尤其是酸性低聚糖可能会影响母乳喂养新生儿的淋巴细胞成熟。
Human milk contains large amounts of free oligosaccharides (HMOs). HMOs have been shown to exert antiinflammatory properties, and evidence for their immunomodulatory effects is increasing. The purpose of this study was to evaluate influences of two human breast milk-derived oligosaccharide samples (neutral and acidic oligosaccharides), and of a low-molecular-weight fucoidan on cytokine production and activation of cord blood mononuclear cells. Cord blood mononuclear cells from randomly chosen healthy newborns were co-cultured with the oligosaccharide samples. By means of flow cytometry, intracellular cytokine production (d 20) and surface marker expression of T cells (d 5) were measured. In vitro-induced Ig levels were quantified nephelometrically (total IgG1) and by ELISA (total IgE) in the supernatant of cell cultures. The acidic oligosaccharide fraction increased the percentage of interferon-gamma producing CD3+CD4+ and CD3+CD8+ cells (p < 0.05) and the IL-13 production in CD3+CD8+ cells (p < 0.05). In acidic oligosaccharide cultures, CD25+ expression on CD3+CD4+ cells was significantly elevated (p < 0.05). Low-molecular-weight fucoidan induced IL-4 production in CD3+CD4+ T cells (p < 0.05) and IL-13 production in CD3+CD8+ T cells (p < 0.05), whereas interferon-gamma production remained unaffected in both T-cell populations. Ig production (total IgE and total IgG1) remained unaffected. Human milk-derived oligosaccharides and plant-derived oligosaccharides affect the cytokine production and activation of cord blood derived T cells in vitro. Therefore, oligosaccharides and, in particular, acidic oligosaccharides may influence lymphocyte maturation in breast-fed newborns.